Mir-370-3p Impairs Glioblastoma Stem-Like Cell Malignancy Regulating a Complex Interplay between HMGA2/HIF1A and the Oncogenic Long Non-Coding RNA (lncRNA) NEAT1
Abstract
:1. Introduction
2. Results
2.1. MiR-370-3p is Downregulated in GBM and Its Restoration Impairs Tumorigenic Ability of GSCs
2.2. Restoration of miR-370-3p Inhibits Tumor Growth in Orthotopic Xenograft Mouse Models
2.3. Tumor Suppressor Function of miR-370-3p Involved Inhibition of EMT and Response to Hypoxia in GSCs
2.4. MiR-370-3p Interacts with NEAT1 and Their Expression is Inversely Correlated in GBM Tissues and GSC Lines
3. Discussion
4. Methods
4.1. GBM and Normal Brain Tissue Sample Collection and Cell Cultures
4.2. RNA Extraction and Quantitative Real-Time PCR (qRT-PCR)
4.3. Flow Cytometry
4.4. Plasmid Constructs and Lentivirus Infection
4.5. Cell Growth, Migration, and Colony Formation Assay
4.6. Orthotopic Xenograft Mouse Models
4.7. Noncoding-RNA Target Prediction
4.8. Reporter Assay
4.9. Gene Array
4.10. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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For: 5′–>3′ | Rev: 5′–>3′ | |
---|---|---|
NEAT1 | TGCTTGTTCCAGAGCCCATGAATGCCA | GTTCTACAGCTTAGGGATCTTCTTGAAGC |
HIF1A | CATCAGCTATTTGCGTGTGAGGA | AGCAATTCATCTGTGCTTTCATGTC |
HMGA2 | AGTCCCTCTAAAGCAGCTCA | GTCCTCTTCGGCAGACTCTT |
GAPDH | ACCTGACCTGCCGTCTAGAAAA | CCTGCTTCACCACCTTCTTGA |
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Lulli, V.; Buccarelli, M.; Ilari, R.; Castellani, G.; De Dominicis, C.; Di Giamberardino, A.; D′Alessandris, Q.G.; Giannetti, S.; Martini, M.; Stumpo, V.; et al. Mir-370-3p Impairs Glioblastoma Stem-Like Cell Malignancy Regulating a Complex Interplay between HMGA2/HIF1A and the Oncogenic Long Non-Coding RNA (lncRNA) NEAT1. Int. J. Mol. Sci. 2020, 21, 3610. https://doi.org/10.3390/ijms21103610
Lulli V, Buccarelli M, Ilari R, Castellani G, De Dominicis C, Di Giamberardino A, D′Alessandris QG, Giannetti S, Martini M, Stumpo V, et al. Mir-370-3p Impairs Glioblastoma Stem-Like Cell Malignancy Regulating a Complex Interplay between HMGA2/HIF1A and the Oncogenic Long Non-Coding RNA (lncRNA) NEAT1. International Journal of Molecular Sciences. 2020; 21(10):3610. https://doi.org/10.3390/ijms21103610
Chicago/Turabian StyleLulli, Valentina, Mariachiara Buccarelli, Ramona Ilari, Giorgia Castellani, Chiara De Dominicis, Alessandra Di Giamberardino, Quintino Giorgio D′Alessandris, Stefano Giannetti, Maurizio Martini, Vittorio Stumpo, and et al. 2020. "Mir-370-3p Impairs Glioblastoma Stem-Like Cell Malignancy Regulating a Complex Interplay between HMGA2/HIF1A and the Oncogenic Long Non-Coding RNA (lncRNA) NEAT1" International Journal of Molecular Sciences 21, no. 10: 3610. https://doi.org/10.3390/ijms21103610