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Open AccessArticle

Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression

Research Division, Incospharm Corporation, Daejeon 34036, Korea
P&K Skin Research Center Company Limited, Seoul 07236, Korea
Research Division, Cha Meditech Corporation, Daejeon 34025, Korea
CHA Bio F&C, Seongnam 13488, Korea
Department of Dermatology, CHA Bundang Medical Center, CHA University, Seongnam 13496, Korea
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 73;
Received: 6 November 2019 / Revised: 15 December 2019 / Accepted: 18 December 2019 / Published: 20 December 2019
(This article belongs to the Special Issue Molecular Mechanisms of Skin Aging)
The dermal-epidermal junction (DEJ) provides a physical and biological interface between the epidermis and the dermis. In addition to providing a structural integrity, the DEJ also acts as a passageway for molecular transport. Based on the recently reported importance of the DEJ in skin aging, novel peptide derivatives have been tested for their effects on basement membrane (BM) protein expressions in cultured human epidermal keratinocytes. As a result, protein expressions of collagen XVII, laminin and nidogen were stimulated by the test peptide and peptides complex. Further ex vivo evaluation using excised human skin, confirmed that the topical application of the peptides complex significantly increased dermal collagen expression, as well as expressions of collagen XVII and laminin. Interestingly, while the origin of the laminin protein is epidermal keratinocytes, the immunohistochemical staining of skin showed that laminin was only detected in the uppermost layer of the dermis, which suggests a tight assembly of laminin protein onto the dermal side of the DEJ. These results suggest that a peptide complex could improve the structural properties of the DEJ through its ability to stimulate BM proteins. In order to evaluate the anti-wrinkle benefits of the peptide complex in vivo, a clinical study was performed on 22 healthy Asian female volunteers older than 40 years. As a result, significant improvements in skin wrinkles for all of the five sites were observed after two weeks, as assessed by skin topographic measurements. Collectively, these results demonstrate the anti-aging efficacy of the peptides complex. View Full-Text
Keywords: dermal-epidermal junction; nidogen; laminin; wrinkle; clinical efficacy; peptides complex dermal-epidermal junction; nidogen; laminin; wrinkle; clinical efficacy; peptides complex
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Jeong, S.; Yoon, S.; Kim, S.; Jung, J.; Kor, M.; Shin, K.; Lim, C.; Han, H.S.; Lee, H.; Park, K.-Y.; Kim, J.; Chung, H.J.; Kim, H.J. Anti-Wrinkle Benefits of Peptides Complex Stimulating Skin Basement Membrane Proteins Expression. Int. J. Mol. Sci. 2020, 21, 73.

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