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Open AccessArticle

Integrative Transcriptomic and Proteomic Analyses of Molecular Mechanism Responding to Salt Stress during Seed Germination in Hulless Barley

1
College of Forestry, Henan Agricultural University, Zhengzhou 450002, China
2
College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016, China
3
Gansu Provincial Key Lab of Aridland Crop Science, Lanzhou 730070, China
4
Gansu Key Lab of Crop Improvement and Germplasm Enhancement, Lanzhou 730070, China
5
State Key Laboratory of Plant Genomics, National Centre for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2020, 21(1), 359; https://doi.org/10.3390/ijms21010359
Received: 3 December 2019 / Revised: 3 January 2020 / Accepted: 3 January 2020 / Published: 6 January 2020
(This article belongs to the Special Issue Plant Proteomic Research 3.0)
Hulless barley (Hordeum vulgare L. var. nudum) is one of the most important crops in the Qinghai-Tibet Plateau. Soil salinity seriously affects its cultivation. To investigate the mechanism of salt stress response during seed germination, two contrasting hulless barley genotypes were selected to first investigate the molecular mechanism of seed salinity response during the germination stage using RNA-sequencing and isobaric tags for relative and absolute quantitation technologies. Compared to the salt-sensitive landrace lk621, the salt-tolerant one lk573 germinated normally under salt stress. The changes in hormone contents also differed between lk621 and lk573. In lk573, 1597 differentially expressed genes (DEGs) and 171 differentially expressed proteins (DEPs) were specifically detected at 4 h after salt stress, and correspondingly, 2748 and 328 specifically detected at 16 h. Most specific DEGs in lk573 were involved in response to oxidative stress, biosynthetic process, protein localization, and vesicle-mediated transport, and most specific DEPs were assigned to an oxidation-reduction process, carbohydrate metabolic process, and protein phosphorylation. There were 96 genes specifically differentially expressed at both transcriptomic and proteomic levels in lk573. These results revealed the molecular mechanism of salt tolerance and provided candidate genes for further study and salt-tolerant improvement in hulless barley. View Full-Text
Keywords: salt stress; seed germination; transcriptome; proteome; hulless barley salt stress; seed germination; transcriptome; proteome; hulless barley
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Lai, Y.; Zhang, D.; Wang, J.; Wang, J.; Ren, P.; Yao, L.; Si, E.; Kong, Y.; Wang, H. Integrative Transcriptomic and Proteomic Analyses of Molecular Mechanism Responding to Salt Stress during Seed Germination in Hulless Barley. Int. J. Mol. Sci. 2020, 21, 359.

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