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Open AccessArticle

Cyclin-Dependent Kinase Inhibitors KRP1 and KRP2 Are Involved in Grain Filling and Seed Germination in Rice (Oryza sativa L.)

1
State Key Lab of Rice Biology, China National Rice Research Institute, Hangzhou 311400, China
2
Biotechnology Unit, National Cereals Research Institute, Badeggi, Bida 912101, Nigeria
3
College of Life Science, Yangtze University, Jingzhou 434025, China
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2020, 21(1), 245; https://doi.org/10.3390/ijms21010245
Received: 21 November 2019 / Revised: 26 December 2019 / Accepted: 26 December 2019 / Published: 30 December 2019
(This article belongs to the Special Issue Plant Cell and Organism Development)
Cyclin-dependent kinase inhibitors known as KRPs (kip-related proteins) control the progression of plant cell cycles and modulate various plant developmental processes. However, the function of KRPs in rice remains largely unknown. In this study, two rice KRPs members, KRP1 and KRP2, were found to be predominantly expressed in developing seeds and were significantly induced by exogenous abscisic acid (ABA) and Brassinosteroid (BR) applications. Sub-cellular localization experiments showed that KRP1 was mainly localized in the nucleus of rice protoplasts. KRP1 overexpression transgenic lines (OxKRP1), krp2 single mutant (crkrp2), and krp1/krp2 double mutant (crkrp1/krp2) all exhibited significantly smaller seed width, seed length, and reduced grain weight, with impaired seed germination and retarded early seedling growth, suggesting that disturbing the normal steady state of KRP1 or KRP2 blocks seed development partly through inhibiting cell proliferation and enlargement during grain filling and seed germination. Furthermore, two cyclin-dependent protein kinases, CDKC;2 and CDKF;3, could interact with KRP1 in a yeast-two-hybrid system, indicating that KRP1 might regulate the mitosis cell cycle and endoreduplication through the two targets. In a word, this study shed novel insights into the regulatory roles of KRPs in rice seed maturation and germination. View Full-Text
Keywords: rice (Oryza sativa L.); cyclin-dependent kinase inhibitors; grain filling; seed germination rice (Oryza sativa L.); cyclin-dependent kinase inhibitors; grain filling; seed germination
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Ajadi, A.A.; Tong, X.; Wang, H.; Zhao, J.; Tang, L.; Li, Z.; Liu, X.; Shu, Y.; Li, S.; Wang, S.; Liu, W.; Tajo, S.M.; Zhang, J.; Wang, Y. Cyclin-Dependent Kinase Inhibitors KRP1 and KRP2 Are Involved in Grain Filling and Seed Germination in Rice (Oryza sativa L.). Int. J. Mol. Sci. 2020, 21, 245.

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