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Open AccessArticle

Experiments with Snails Add to Our Knowledge about the Role of aPKC Subfamily Kinases in Learning

1
Cellular Neurobiology of Learning Lab, Institute of Higher Nervous Activity and Neurophysiology, Russian Academy of Sciences, Moscow 117485, Russia
2
Department of Translational Medicine, Pirogov Russian National Research Medical University, Moscow 117997, Russia
3
Laboratory of Cell Biology, Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Moscow 119334, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(9), 2117; https://doi.org/10.3390/ijms20092117
Received: 26 March 2019 / Revised: 26 April 2019 / Accepted: 26 April 2019 / Published: 29 April 2019
(This article belongs to the Special Issue Kinase Signal Transduction 2019)
Protein kinase Mζ is considered important for memory formation and maintenance in different species, including invertebrates. PKMζ participates in multiple molecular pathways in neurons, regulating translation initiation rate, AMPA receptors turnover, synaptic scaffolding assembly, and other processes. Here, for the first time, we established the sequence of mRNA encoding PKMζ homolog in land snail Helix lucorum. We annotated important features of this mRNA: domains, putative capping sites, translation starts, and splicing sites. We discovered that this mRNA has at least two isoforms, and one of them lacks sequence encoding C1 domain. C1 deletion may be unique for snail because it has not been previously found in other species. We performed behavioral experiments with snails, measured expression levels of identified isoforms, and confirmed that their expression correlates with one type of learning. View Full-Text
Keywords: atypical PKCs; PKMζ; mollusks; Helix lucorum; mRNA isoforms; mRNA expression; 5′-RACE; learning and memory atypical PKCs; PKMζ; mollusks; Helix lucorum; mRNA isoforms; mRNA expression; 5′-RACE; learning and memory
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Chesnokova, E.; Zuzina, A.; Bal, N.; Vinarskaya, A.; Roshchin, M.; Artyuhov, A.; Dashinimaev, E.; Aseyev, N.; Balaban, P.; Kolosov, P. Experiments with Snails Add to Our Knowledge about the Role of aPKC Subfamily Kinases in Learning. Int. J. Mol. Sci. 2019, 20, 2117.

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