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Article

PHB is Produced from Glycogen Turn-over during Nitrogen Starvation in Synechocystis sp. PCC 6803

1
Interfaculty Institute of Microbiology and Infection Medicine Tübingen, Eberhard-Karls-Universität Tübingen, 72076 Tübingen, Germany
2
Department of Biology, Botanical Institute, Christian-Albrechts-University, 24118 Kiel, Germany
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(8), 1942; https://doi.org/10.3390/ijms20081942
Received: 4 April 2019 / Revised: 17 April 2019 / Accepted: 18 April 2019 / Published: 20 April 2019
(This article belongs to the Special Issue Biopolymer Composites 2019)
Polyhydroxybutyrate (PHB) is a polymer of great interest as a substitute for conventional plastics, which are becoming an enormous environmental problem. PHB can be produced directly from CO2 in photoautotrophic cyanobacteria. The model cyanobacterium Synechocystis sp. PCC 6803 produces PHB under conditions of nitrogen starvation. However, it is so far unclear which metabolic pathways provide the precursor molecules for PHB synthesis during nitrogen starvation. In this study, we investigated if PHB could be derived from the main intracellular carbon pool, glycogen. A mutant of the major glycogen phosphorylase, GlgP2 (slr1367 product), was almost completely impaired in PHB synthesis. Conversely, in the absence of glycogen synthase GlgA1 (sll0945 product), cells not only produced less PHB, but were also impaired in acclimation to nitrogen depletion. To analyze the role of the various carbon catabolic pathways (EMP, ED and OPP pathways) for PHB production, mutants of key enzymes of these pathways were analyzed, showing different impact on PHB synthesis. Together, this study clearly indicates that PHB in glycogen-producing Synechocystis sp. PCC 6803 cells is produced from this carbon-pool during nitrogen starvation periods. This knowledge can be used for metabolic engineering to get closer to the overall goal of a sustainable, carbon-neutral bioplastic production. View Full-Text
Keywords: cyanobacteria; bioplastic; PHB; sustainable; glycogen; metabolic engineering; Synechocystis cyanobacteria; bioplastic; PHB; sustainable; glycogen; metabolic engineering; Synechocystis
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MDPI and ACS Style

Koch, M.; Doello, S.; Gutekunst, K.; Forchhammer, K. PHB is Produced from Glycogen Turn-over during Nitrogen Starvation in Synechocystis sp. PCC 6803. Int. J. Mol. Sci. 2019, 20, 1942. https://doi.org/10.3390/ijms20081942

AMA Style

Koch M, Doello S, Gutekunst K, Forchhammer K. PHB is Produced from Glycogen Turn-over during Nitrogen Starvation in Synechocystis sp. PCC 6803. International Journal of Molecular Sciences. 2019; 20(8):1942. https://doi.org/10.3390/ijms20081942

Chicago/Turabian Style

Koch, Moritz, Sofía Doello, Kirstin Gutekunst, and Karl Forchhammer. 2019. "PHB is Produced from Glycogen Turn-over during Nitrogen Starvation in Synechocystis sp. PCC 6803" International Journal of Molecular Sciences 20, no. 8: 1942. https://doi.org/10.3390/ijms20081942

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