Comparison of Proton and Photon Beam Irradiation in Radiation-Induced Intestinal Injury Using a Mouse Model
Abstract
:1. Introduction
2. Results
2.1. High Doses of Therapeutic Radiation Reduce the Number of Crypts in the Mouse Intestine
2.2. Proton Irradiation Leads to a Higher Decrease in the Numbers of Surviving Crypts in the Jejunum versus X-Ray Irradiation
2.3. Proton Irradiation Leads to a Higher Decrease in Quiescent Stem Cells and Progenitor Cells in the Jejunum versus X-Ray Irradiation
2.4. Proton Irradiation Leads to a Higher Rate of Apoptotic Cell Death in the Jejunum Crypts versus X-Ray Irradiation
3. Discussion
4. Materials and Methods
4.1. Mice and Irradiation Procedures
4.2. Crypt Microcolony Assay
4.3. Edu Assay
4.4. Immunohistochemistry
4.5. TUNEL Assay
4.6. Statistics
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
EdU | 5-ethynyl-2′-deoxyuridine |
GI | gastrointestinal |
H&E | haematoxylin and eosin |
ISC | intestinal stem cell |
IHC | immunohistochemistry |
OLFM4 | Olfactomedin 4 |
PBT | proton beam therapy |
RBE | relative biological effectiveness |
RT | radiation therapy |
SOBP | spread-out-Bragg-peak |
TUNEL | terminal deoxynucleotidyl transferase-mediated dUTP nick-end labelling |
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Choi, C.; Lee, C.; Shin, S.-W.; Kim, S.-Y.; Hong, S.N.; Park, H.C. Comparison of Proton and Photon Beam Irradiation in Radiation-Induced Intestinal Injury Using a Mouse Model. Int. J. Mol. Sci. 2019, 20, 1894. https://doi.org/10.3390/ijms20081894
Choi C, Lee C, Shin S-W, Kim S-Y, Hong SN, Park HC. Comparison of Proton and Photon Beam Irradiation in Radiation-Induced Intestinal Injury Using a Mouse Model. International Journal of Molecular Sciences. 2019; 20(8):1894. https://doi.org/10.3390/ijms20081894
Chicago/Turabian StyleChoi, Changhoon, Chansu Lee, Sung-Won Shin, Shin-Yeong Kim, Sung Noh Hong, and Hee Chul Park. 2019. "Comparison of Proton and Photon Beam Irradiation in Radiation-Induced Intestinal Injury Using a Mouse Model" International Journal of Molecular Sciences 20, no. 8: 1894. https://doi.org/10.3390/ijms20081894