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Article

Low-Luminance Blue Light-Enhanced Phototoxicity in A2E-Laden RPE Cell Cultures and Rats

1
School of Pharmacy, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan
2
Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan
3
Institute of Drug Education and Research, College of Pharmacy, University of Houston, Texas 77004, USA
4
Department of Pharmacology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
5
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(7), 1799; https://doi.org/10.3390/ijms20071799
Received: 21 February 2019 / Revised: 30 March 2019 / Accepted: 8 April 2019 / Published: 11 April 2019
N-retinylidene-N-retinylethanolamine (A2E) and other bisretinoids are components of lipofuscin and accumulate in retinal pigment epithelial (RPE) cells—these adducts are recognized in the pathogenesis of retinal degeneration. Further, blue light-emitting diode (LED) light (BLL)-induced retinal toxicity plays an important role in retinal degeneration. Here, we demonstrate that low-luminance BLL enhances phototoxicity in A2E-laden RPE cells and rats. RPE cells were subjected to synthetic A2E, and the effects of BLL on activation of apoptotic biomarkers were examined by measuring the levels of cleaved caspase-3. BLL modulates the protein expression of zonula-occludens 1 (ZO-1) and paracellular permeability in A2E-laden RPE cells. Early inflammatory and angiogenic genes were also screened after short-term BLL exposure. In this study, we developed a rat model for A2E treatment with or without BLL exposure for 21 days. BLL exposure caused fundus damage, decreased total retinal thickness, and caused neuron transduction injury in the retina, which were consistent with the in vitro data. We suggest that the synergistic effects of BLL and A2E accumulation in the retina increase the risk of retinal degeneration. These outcomes help elucidate the associations between BLL/A2E and angiogenic/apoptotic mechanisms, as well as furthering therapeutic strategies. View Full-Text
Keywords: blue light; N-retinylidene-N-retinylethanolamine (A2E); retinal pigment epithelial cells (RPE); retinal damage blue light; N-retinylidene-N-retinylethanolamine (A2E); retinal pigment epithelial cells (RPE); retinal damage
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MDPI and ACS Style

Lin, C.-H.; Wu, M.-R.; Huang, W.-J.; Chow, D.S.-L.; Hsiao, G.; Cheng, Y.-W. Low-Luminance Blue Light-Enhanced Phototoxicity in A2E-Laden RPE Cell Cultures and Rats. Int. J. Mol. Sci. 2019, 20, 1799. https://doi.org/10.3390/ijms20071799

AMA Style

Lin C-H, Wu M-R, Huang W-J, Chow DS-L, Hsiao G, Cheng Y-W. Low-Luminance Blue Light-Enhanced Phototoxicity in A2E-Laden RPE Cell Cultures and Rats. International Journal of Molecular Sciences. 2019; 20(7):1799. https://doi.org/10.3390/ijms20071799

Chicago/Turabian Style

Lin, Cheng-Hui, Man-Ru Wu, Wei-Jan Huang, Diana S.-L. Chow, George Hsiao, and Yu-Wen Cheng. 2019. "Low-Luminance Blue Light-Enhanced Phototoxicity in A2E-Laden RPE Cell Cultures and Rats" International Journal of Molecular Sciences 20, no. 7: 1799. https://doi.org/10.3390/ijms20071799

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