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Volume 20
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Article

Rosiglitazone Enhances Browning Adipocytes in Association with MAPK and PI3-K Pathways During the Differentiation of Telomerase-Transformed Mesenchymal Stromal Cells into Adipocytes

by
Abeer Maher Fayyad
1,
Amir Ali Khan
1,2,*,
Sallam Hasan Abdallah
2,
Sara Sultan Alomran
1,
Khalid Bajou
1,2 and
Muhammad Nasir Khan Khattak
1
1
Department of Applied Biology, College of Sciences, University of Sharjah, Sharjah 27272, UAE
2
Human Genetics & Stem cells research group, Research Institute of Sciences & Engineering, University of Sharjah, Sharjah 27272, UAE
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(7), 1618; https://doi.org/10.3390/ijms20071618
Submission received: 17 February 2019 / Revised: 20 March 2019 / Accepted: 29 March 2019 / Published: 1 April 2019
(This article belongs to the Section Molecular Biology)
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Abstract

Obesity is a major risk for diabetes. Brown adipose tissue (BAT) mediates production of heat while white adipose tissue (WAT) function in the storage of fat. Roles of BAT in the treatment of obesity and related disorders warrants more investigation. Peroxisome proliferator activator receptor gamma (PPAR-γ) is the master regulator of both BAT and WAT adipogenesis and has roles in glucose and fatty acid metabolism. Adipose tissue is the major expression site for PPAR-γ. In this study, the effects of rosiglitazone on the brown adipogenesis and the association of MAPK and PI3K pathways was investigated during the in vitro adipogenic differentiation of telomerase transformed mesenchymal stromal cells (iMSCs). Our data indicate that 2 µM rosiglitazone enhanced adipogenesis by over-expression of PPAR-γ and C/EBP-α. More specifically, brown adipogenesis was enhanced by the upregulation of EBF2 and UCP-1 and evidenced by multilocular fatty droplets morphology of the differentiated adipocytes. We also found that rosiglitazone significantly activated MAPK and PI3K pathways at the maturation stage of differentiation. Overall, the results indicate that rosiglitazone induced overexpression of PPAR-γ that in turn enhanced adipogenesis, particularly browning adipogenesis. This study reports the browning effects of rosiglitazone during the differentiation of iMSCs into adipocytes in association with the activation of MAPK and PI3K signaling pathways.
Keywords: rosiglitazone; telomerase-transformed mesenchymal stromal cells (iMSC3); brown adipocytes; PPAR-γ; UCP-1; PI-3 kinase pathway; MAP kinase pathway rosiglitazone; telomerase-transformed mesenchymal stromal cells (iMSC3); brown adipocytes; PPAR-γ; UCP-1; PI-3 kinase pathway; MAP kinase pathway

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MDPI and ACS Style

Fayyad, A.M.; Khan, A.A.; Abdallah, S.H.; Alomran, S.S.; Bajou, K.; Khattak, M.N.K. Rosiglitazone Enhances Browning Adipocytes in Association with MAPK and PI3-K Pathways During the Differentiation of Telomerase-Transformed Mesenchymal Stromal Cells into Adipocytes. Int. J. Mol. Sci. 2019, 20, 1618. https://doi.org/10.3390/ijms20071618

AMA Style

Fayyad AM, Khan AA, Abdallah SH, Alomran SS, Bajou K, Khattak MNK. Rosiglitazone Enhances Browning Adipocytes in Association with MAPK and PI3-K Pathways During the Differentiation of Telomerase-Transformed Mesenchymal Stromal Cells into Adipocytes. International Journal of Molecular Sciences. 2019; 20(7):1618. https://doi.org/10.3390/ijms20071618

Chicago/Turabian Style

Fayyad, Abeer Maher, Amir Ali Khan, Sallam Hasan Abdallah, Sara Sultan Alomran, Khalid Bajou, and Muhammad Nasir Khan Khattak. 2019. "Rosiglitazone Enhances Browning Adipocytes in Association with MAPK and PI3-K Pathways During the Differentiation of Telomerase-Transformed Mesenchymal Stromal Cells into Adipocytes" International Journal of Molecular Sciences 20, no. 7: 1618. https://doi.org/10.3390/ijms20071618

APA Style

Fayyad, A. M., Khan, A. A., Abdallah, S. H., Alomran, S. S., Bajou, K., & Khattak, M. N. K. (2019). Rosiglitazone Enhances Browning Adipocytes in Association with MAPK and PI3-K Pathways During the Differentiation of Telomerase-Transformed Mesenchymal Stromal Cells into Adipocytes. International Journal of Molecular Sciences, 20(7), 1618. https://doi.org/10.3390/ijms20071618

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