Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption

Ikarashi, Nobutomo; Nagoya, Chika; Kon, Risako; Kitaoka, Satoshi; Kajiwara, Sayuri; Saito, Masayo; Kawabata, Akane; Ochiai, Wataru; Sugiyama, Kiyoshi

doi:10.3390/ijms20071559

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Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption

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Department of Biomolecular Pharmacology, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan

Department of Clinical Pharmacokinetics, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan

Department of Functional Molecular Kinetics, Hoshi University, 2-4-41 Ebara, Shinagawa-ku, Tokyo 142-8501, Japan

Authors to whom correspondence should be addressed.

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These authors contributed equally to this work.

Int. J. Mol. Sci. 2019, 20(7), 1559; https://doi.org/10.3390/ijms20071559

Received: 6 March 2019 / Revised: 25 March 2019 / Accepted: 26 March 2019 / Published: 28 March 2019

(This article belongs to the Special Issue Aquaporins: Water Channels Essential for Living Organisms 2.0)

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Abstract

Aquaporin-3 (AQP3) plays an important role in water transport in the gastrointestinal (GI) tract. In this study, we conducted a Caco-2 cell permeability assay to examine how changes in the expression and function of AQP3 affect the rate at which a drug is absorbed via passive transport in the GI tract. When the function of AQP3 was inhibited by mercuric chloride or phloretin, there was no change in warfarin permeability. In contrast, when the expression of AQP3 protein was decreased by prostaglandin E₂ (PGE₂) treatment, warfarin permeability increased to approximately twice the control level, and membrane fluidity increased by 15%. In addition, warfarin permeability increased to an extent comparable to that after PGE₂ treatment when cell membrane fluidity was increased by 10% via boric acid/EDTA treatment. These findings suggest the possibility that the increased drug absorption under decreased AQP3 expression was attributable to increased membrane fluidity. The results of this study demonstrate that the rate of water transport has little effect on drug absorption. However, our findings also indicate that although AQP3 and other similar transmembrane proteins do not themselves transport drugs, changes in their expression levels can cause changes in cell membrane fluidity, thus affecting drug absorption rates. View Full-Text

Keywords: aquaporin; Caco-2 cell; membrane fluidity; drug absorption

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MDPI and ACS Style

Ikarashi, N.; Nagoya, C.; Kon, R.; Kitaoka, S.; Kajiwara, S.; Saito, M.; Kawabata, A.; Ochiai, W.; Sugiyama, K. Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption. Int. J. Mol. Sci. 2019, 20, 1559. https://doi.org/10.3390/ijms20071559

AMA Style

Ikarashi N, Nagoya C, Kon R, Kitaoka S, Kajiwara S, Saito M, Kawabata A, Ochiai W, Sugiyama K. Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption. International Journal of Molecular Sciences. 2019; 20(7):1559. https://doi.org/10.3390/ijms20071559

Chicago/Turabian Style

Ikarashi, Nobutomo, Chika Nagoya, Risako Kon, Satoshi Kitaoka, Sayuri Kajiwara, Masayo Saito, Akane Kawabata, Wataru Ochiai, and Kiyoshi Sugiyama. 2019. "Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption" International Journal of Molecular Sciences 20, no. 7: 1559. https://doi.org/10.3390/ijms20071559

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Changes in the Expression of Aquaporin-3 in the Gastrointestinal Tract Affect Drug Absorption

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