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Adiponectin—Consideration for its Role in Skeletal Muscle Health
Article

Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity

1
Department of Biomedical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan
2
Morinaga Institute of Biological Science, Yokohama 236-0003, Japan
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(7), 1541; https://doi.org/10.3390/ijms20071541
Received: 2 March 2019 / Revised: 22 March 2019 / Accepted: 25 March 2019 / Published: 27 March 2019
(This article belongs to the Special Issue Mechanisms of Adiponectin Action)
Both adiponectin and secreted protein, acidic and rich in cysteine (SPARC) inhibit platelet-derived growth factor-BB (PDGF-BB)-induced and basic fibroblast growth factor (FGF2)-induced angiogenic activities through direct and indirect interactions. Although SPARC enhances nerve growth factor (NGF)-dependent neurogenesis, the physical interaction of NGFβ with adiponectin and SPARC remains obscure. Therefore, we first examined their intermolecular interaction by surface plasmon resonance method. NGFβ bound to immobilized SPARC with the binding constant of 59.4 nM, comparable with that of PDGF-BB (24.5 nM) but far less than that of FGF2 (14.4 µM). NGFβ bound to immobilized full length adiponectin with the binding constant of 103 nM, slightly higher than those of PDGF-BB (24.3 nM) and FGF2 (80.2 nM), respectively. Treatment of PC12 cells with SPARC did not cause mitogen-activated protein kinase (MAPK) activation and neurite outgrowth. However, simultaneous addition of SPARC with NGFβ enhanced NGFβ-induced MAPK phosphorylation and neurite outgrowth. Treatment of the cells with adiponectin increased AMP-activated protein kinase (AMPK) phosphorylation but failed to induce neurite outgrowth. Simultaneous treatment with NGFβ and adiponectin significantly reduced cell size and the number of cells with neurite, even after silencing the adiponectin receptors by their siRNA. These results indicate that NGFβ directly interacts with adiponectin and SPARC, whereas these interactions oppositely regulate NGFβ functions. View Full-Text
Keywords: adiponectin; AMPK; BIAcore; extracellular signal-regulated kinase (ERK); matricellular proteins; neuritogenesis; NGFβ; PC12 cells; Secreted protein; acidic and rich in cysteine (SPARC) adiponectin; AMPK; BIAcore; extracellular signal-regulated kinase (ERK); matricellular proteins; neuritogenesis; NGFβ; PC12 cells; Secreted protein; acidic and rich in cysteine (SPARC)
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MDPI and ACS Style

Okura, Y.; Imao, T.; Murashima, S.; Shibata, H.; Kamikavwa, A.; Okamatsu-Ogura, Y.; Saito, M.; Kimura, K. Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity. Int. J. Mol. Sci. 2019, 20, 1541. https://doi.org/10.3390/ijms20071541

AMA Style

Okura Y, Imao T, Murashima S, Shibata H, Kamikavwa A, Okamatsu-Ogura Y, Saito M, Kimura K. Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity. International Journal of Molecular Sciences. 2019; 20(7):1541. https://doi.org/10.3390/ijms20071541

Chicago/Turabian Style

Okura, Yuu, Takeshi Imao, Seisuke Murashima, Haruki Shibata, Akihiro Kamikavwa, Yuko Okamatsu-Ogura, Masayuki Saito, and Kazuhiro Kimura. 2019. "Interaction of Nerve Growth Factor β with Adiponectin and SPARC Oppositely Modulates its Biological Activity" International Journal of Molecular Sciences 20, no. 7: 1541. https://doi.org/10.3390/ijms20071541

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