Effects of Pressure and Temperature on the Atomic Fluctuations of Dihydrofolate Reductase from a Psychropiezophile and a Mesophile
Abstract
:1. Introduction
2. Results and Discussion
3. Materials and Methods
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Protein (T, K; P, bar) | MSF (Å2) * | Vapp/NHA (Å3) * | Nw,in | NHB | τHB (ps) |
---|---|---|---|---|---|
EcDHFR (279; 1) | 0.65 ± 0.02 | 17.38 ± 0.02 | 1.96 ± 0.31 | 107 ± 2 | 27.7 ± 3.4 |
MpDHFR (279; 1) | 0.76 ± 0.08 | 17.73 ± 0.04 | 3.25 ± 0.30 | 103 ± 2 | 30.6 ± 3.7 |
EcDHFR (279; 220) | 0.71 ± 0.11 | 17.55 ± 0.01 | 2.08 ± 0.31 | 105 ± 1 | 25.5 ± 2.7 |
MpDHFR (279; 220) | 0.83 ± 0.04 | 17.77 ± 0.03 | 3.28 ± 0.40 | 104 ± 1 | 22.5 ± 1.8 |
EcDHFR (310; 1) | 0.93 ± 0.07 | 17.87 ± 0.02 | 2.36 ± 0.37 | 106 ± 1 | 11.6 ± 1.2 |
MpDHFR (310; 1) | 1.39 ± 0.15 | 18.26 ± 0.02 | 4.21 ± 0.32 | 101 ± 2 | 11.2 ± 0.6 |
EcDHFR (310; 220) | 1.04 ± 0.14 | 17.82 ± 0.01 | 2.47 ± 0.36 | 104 ± 1 | 11.2 ± 1.1 |
MpDHFR (310; 220) | 1.49 ± 0.16 | 18.25 ± 0.01 | 3.26 ± 0.42 | 101 ± 2 | 11.1 ± 1.1 |
Protein (P, bar) | ΔT%MSF * | ΔT%Vapp/NHA * | ΔTNw,in | ΔTNHB | ΔT%τHB |
---|---|---|---|---|---|
EcDHFR (1) | 43 | 3 | 0.40 | −1 | −58 |
EcDHFR (220) | 46 | 2 | 0.39 | −1 | −56 |
MpDHFR (1) | 83 | 3 | 0.96 | −2 | −63 |
MpDHFR (220) | 80 | 3 | −0.02 | −3 | −51 |
Protein (T, K) | ΔP%MSF * | ΔP%Vapp/NHA* | ΔPNw,in | ΔPNHB | ΔP%τHB |
---|---|---|---|---|---|
EcDHFR (279) | 9 | 1 | 0.12 | −2 | −8 |
EcDHFR (310) | 12 | 0 | 0.11 | −2 | −3 |
MpDHFR (279) | 9 | 0 | 0.03 | 1 | −26 |
MpDHFR (310) | 7 | 0 | −0.95 | 0 | −1 |
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Huang, Q.; Rodgers, J.M.; Hemley, R.J.; Ichiye, T. Effects of Pressure and Temperature on the Atomic Fluctuations of Dihydrofolate Reductase from a Psychropiezophile and a Mesophile. Int. J. Mol. Sci. 2019, 20, 1452. https://doi.org/10.3390/ijms20061452
Huang Q, Rodgers JM, Hemley RJ, Ichiye T. Effects of Pressure and Temperature on the Atomic Fluctuations of Dihydrofolate Reductase from a Psychropiezophile and a Mesophile. International Journal of Molecular Sciences. 2019; 20(6):1452. https://doi.org/10.3390/ijms20061452
Chicago/Turabian StyleHuang, Qi, Jocelyn M. Rodgers, Russell J. Hemley, and Toshiko Ichiye. 2019. "Effects of Pressure and Temperature on the Atomic Fluctuations of Dihydrofolate Reductase from a Psychropiezophile and a Mesophile" International Journal of Molecular Sciences 20, no. 6: 1452. https://doi.org/10.3390/ijms20061452