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An Integrative Evaluation Method for the Biological Safety of Down and Feather Materials

1
Kawada Feather Co., Ltd., Nagoya, Aichi 453-0064, Japan
2
UMOU Science Lab, Mastusaka, Mie 515-0074, Japan
3
Department of Life Sciences, Graduate School of Bioresources, Mie University, Tsu, Mie 514-8507, Japan
4
Department of Bioinformatics, Mie University Advanced Science Research Promotion Center, Tsu, Mie 514-8507, Japan
5
Mie University Zebrafish Drug Screening Center, Tsu, Mie 514-8507, Japan
6
Graduate School of Regional Innovation Studies, Mie University, Tsu, Mie 514-8507, Japan
7
Boken Quality Evaluation Institute, Minato-Ku, Osaka 552-0021, Japan
8
Department of Chemistry for Materials, Graduate School of Engineering, Mie University, Tsu, Mie 514-8507, Japan
9
Department of Integrative Pharmacology, Mie University Graduate School of Medicine, Tsu, Mie 514-8507, Japan
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(6), 1434; https://doi.org/10.3390/ijms20061434
Received: 28 January 2019 / Revised: 15 March 2019 / Accepted: 18 March 2019 / Published: 21 March 2019
(This article belongs to the Special Issue Zebrafish 2.0: A Model for Toxicological Research)
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Abstract

Background: Down and feather materials have been commonly used and promoted as natural stuffing for warm clothing and bedding. These materials tend to become more allergenic as they become contaminated with microorganisms, in addition to being subjected to several kinds of chemical treatments. The biological or chemical contaminants in these materials pose a major risk to human health, to consumers and manufacturers alike. Here, we report the development of an integrative evaluation method for down and feather materials to assess bacterial contamination and in vivo toxicity. Methods: To assess bacterial contamination, we quantified 16S ribosomal RNA, performed culture tests, and established a conversion formula. To determine in vivo toxicity, we performed a zebrafish embryo toxicity testing (ZFET). Results: Washing the material appropriately decreases the actual number of bacteria in the down and feather samples; in addition, after washing, 16S rRNA sequencing revealed that the bacterial compositions were similar to those in rinse water. The ZFET results showed that even materials with low bacterial contamination showed high toxicity or high teratogenicity, probably because of the presence of unknown chemical additives. Conclusions: We established an integrative evaluation method for down and feather safety, based on bacterial contamination with in vivo toxicity testing. View Full-Text
Keywords: allergy; microbiome; pollution; environment; clothes; bedding allergy; microbiome; pollution; environment; clothes; bedding
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Kawada, T.; Kuroyanagi, J.; Okazaki, F.; Taniguchi, M.; Nakayama, H.; Suda, N.; Abiko, S.; Kaneco, S.; Nishimura, N.; Shimada, Y. An Integrative Evaluation Method for the Biological Safety of Down and Feather Materials. Int. J. Mol. Sci. 2019, 20, 1434.

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