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Time-Resolved Macromolecular Crystallography at Pulsed X-ray Sources

Physics Department, University of Wisconsin-Milwaukee, Milwaukee, WI 53211, USA
Int. J. Mol. Sci. 2019, 20(6), 1401; https://doi.org/10.3390/ijms20061401
Received: 27 February 2019 / Revised: 14 March 2019 / Accepted: 18 March 2019 / Published: 20 March 2019
The focus of structural biology is shifting from the determination of static structures to the investigation of dynamical aspects of macromolecular function. With time-resolved macromolecular crystallography (TRX), intermediates that form and decay during the macromolecular reaction can be investigated, as well as their reaction dynamics. Time-resolved crystallographic methods were initially developed at synchrotrons. However, about a decade ago, extremely brilliant, femtosecond-pulsed X-ray sources, the free electron lasers for hard X-rays, became available to a wider community. TRX is now possible with femtosecond temporal resolution. This review provides an overview of methodological aspects of TRX, and at the same time, aims to outline the frontiers of this method at modern pulsed X-ray sources. View Full-Text
Keywords: time-resolved crystallography; serial femtosecond crystallography; Monte Carlo integration; beta-lactamase; bacterial phytochromes time-resolved crystallography; serial femtosecond crystallography; Monte Carlo integration; beta-lactamase; bacterial phytochromes
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Schmidt, M. Time-Resolved Macromolecular Crystallography at Pulsed X-ray Sources. Int. J. Mol. Sci. 2019, 20, 1401.

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