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Open AccessArticle

Novel 8-Substituted Coumarins That Selectively Inhibit Human Carbonic Anhydrase IX and XII

Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Yeditepe University, Kayisdagi Cad., 34755 Istanbul, Turkey
NEUROFARBA Department, Pharmaceutical and Nutraceutical Section, University of Florence, 50019 Sesto Fiorentino, Italy
Department of Pharmacy, University of Pisa, 56126 Pisa, Italy
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(5), 1208;
Received: 15 February 2019 / Revised: 4 March 2019 / Accepted: 6 March 2019 / Published: 10 March 2019
(This article belongs to the Special Issue Protease and Carbonic Anhydrase Inhibitors, II)
A novel series of 8-substituted coumarin-based compounds, characterized by the presence of alkylpiperazine and arylpiperazine chains, were synthesized and tested for their inhibitory activity against four human carbonic anhydrase (hCA) isoforms. All compounds displayed nanomolar potency against the cancer-related hCA IX and hCA XII; moreover, they were shown to be devoid of any inhibitory activity toward the cytosolic hCA I and hCA II up to 10 µM concentration in the assay system. Therefore, the synthesized coumarin ligands demonstrated to be potent and selective hCA IX/XII inhibitors, and were shown to be as potent as the reference inhibitor acetazolamide against hCA XII, with single-digit nanomolar Ki values. Molecular modeling studies provided a rationale for explaining the selectivity profile of these non-classic hCA inhibitors and their interactions with the enzymes, according to their specific mechanism of action, thus paving the way for future structure-based lead optimization studies. View Full-Text
Keywords: carbonic anhydrase inhibitor; metalloenzymes; coumarins; docking; molecular modeling carbonic anhydrase inhibitor; metalloenzymes; coumarins; docking; molecular modeling
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MDPI and ACS Style

Buran, K.; Bua, S.; Poli, G.; Önen Bayram, F.E.; Tuccinardi, T.; Supuran, C.T. Novel 8-Substituted Coumarins That Selectively Inhibit Human Carbonic Anhydrase IX and XII. Int. J. Mol. Sci. 2019, 20, 1208.

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