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Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-α Ratio in Tears of Patients with Perennial Allergic Conjunctivitis: A Preliminary Study

1
Departamento de Inmunología, ENCB, Instituto Politécnico Nacional, 11340 Ciudad de México, Mexico
2
Department of Immunology and Research Unit, Institute of Ophthalmology “Conde de Valenciana Foundation”, 06800 Mexico City, Mexico
3
Cornea and Refractive Surgery Department, Institute of Ophthalmology “Conde de Valenciana Foundation”, 06800 Mexico City, Mexico
4
Department of Biochemistry, Faculty of Medicine, National Autonomous University of Mexico. P.O. Box 70159, 04510 Mexico City, Mexico
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(5), 1035; https://doi.org/10.3390/ijms20051035
Received: 6 January 2019 / Revised: 12 February 2019 / Accepted: 22 February 2019 / Published: 27 February 2019
(This article belongs to the Special Issue The Interleukins in Health and Disease 2019)
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Abstract

Allergic conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset of IL-10-expressing B cells is involved in inflammatory allergic diseases. In this study, we aimed to evaluate the potential involvement of blood Bregs cells in perennial allergic conjunctivitis (PAC), and interleukins (IL)-1β, IL-6, IL-8, IL-10, and IL-12, and tumor necrosis factor (TNF)-α, were measured in tear samples and compared with healthy controls (HC) using flow cytometry. Non-significant differences in CD19+IL-10+ cell frequency between PAC patients and healthy controls (HC) were observed. Nevertheless, when we analyzed the mean fluorescence intensity (MFI) of IL-10 on CD19+CD38Lo/Med/Hi-gated cells, we observed a significant decrease in MFI in all Bregs subsets in PAC patients. Additionally, tear cytokines showed 2.8 times lower levels of IL-10 than TNF-α in PAC patients when compared to HC. Our findings demonstrate an immunological dysregulation in patients with allergic conjunctivitis, characterized by the low expression of IL-10 in circulating CD19+CD38+ Bregs subsets and an inverted tear IL-10/TNF-α ratio, promoting a local pro-inflammatory microenvironment. These findings highlight the novel pathologic changes involved in ocular allergic diseases. Understanding systemic and local mechanisms will aid the design of immunomodulating therapeutics at different levels. View Full-Text
Keywords: IL-10; TNF-α; tears; Bregs; allergic conjunctivitis IL-10; TNF-α; tears; Bregs; allergic conjunctivitis
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Salazar, A.; Casanova-Méndez, I.; Pacheco-Quito, M.; Velázquez-Soto, H.; Ayala-Balboa, J.; Graue-Hernández, E.O.; Serafín-López, J.; Jiménez-Martínez, M.C. Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-α Ratio in Tears of Patients with Perennial Allergic Conjunctivitis: A Preliminary Study. Int. J. Mol. Sci. 2019, 20, 1035.

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