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Int. J. Mol. Sci. 2019, 20(4), 825; https://doi.org/10.3390/ijms20040825

Proteomic Analysis of the Effect of Inorganic and Organic Chemicals on Silver Nanoparticles in Wheat

1
Faculty of Life and Environmental and Information Sciences, Fukui University of Technology, Fukui 910-8505, Japan
2
Department of Agronomy, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan
3
Department of Botany, PMAS-Arid Agriculture University, Rawalpindi 46300, Pakistan
4
Department of Botany, Women University, Swabi 23340, Pakistan
5
Institute for Comprehensive Medical Science, Fujita Health University, Toyoake 470-1192, Japan
*
Author to whom correspondence should be addressed.
Received: 16 December 2018 / Revised: 4 February 2019 / Accepted: 7 February 2019 / Published: 14 February 2019
(This article belongs to the Special Issue Plant Proteomic Research 2.0)
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Abstract

Production and utilization of nanoparticles (NPs) are increasing due to their positive and stimulating effects on biological systems. Silver (Ag) NPs improve seed germination, photosynthetic efficiency, plant growth, and antimicrobial activities. In this study, the effects of chemo-blended Ag NPs on wheat were investigated using the gel-free/label-free proteomic technique. Morphological analysis revealed that chemo-blended Ag NPs resulted in the increase of shoot length, shoot fresh weight, root length, and root fresh weight. Proteomic analysis indicated that proteins related to photosynthesis and protein synthesis were increased, while glycolysis, signaling, and cell wall related proteins were decreased. Proteins related to redox and mitochondrial electron transport chain were also decreased. Glycolysis associated proteins such as glyceraldehyde-3-phosphate dehydrogenase increased as well as decreased, while phosphoenol pyruvate carboxylase was decreased. Antioxidant enzyme activities such as superoxide dismutase, catalase, and peroxidase were promoted in response to the chemo-blended Ag NPs. These results suggested that chemo-blended Ag NPs promoted plant growth and development through regulation of energy metabolism by suppression of glycolysis. Number of grains/spike, 100-grains weight, and yield of wheat were stimulated with chemo-blended Ag NPs. Morphological study of next generational wheat plants depicted normal growth, and no toxic effects were observed. Therefore, morphological, proteomic, yield, and next generation results revealed that chemo-blended Ag NPs may promote plant growth and development through alteration in plant metabolism. View Full-Text
Keywords: proteomics; wheat; silver nanoparticles proteomics; wheat; silver nanoparticles
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Jhanzab, H.M.; Razzaq, A.; Bibi, Y.; Yasmeen, F.; Yamaguchi, H.; Hitachi, K.; Tsuchida, K.; Komatsu, S. Proteomic Analysis of the Effect of Inorganic and Organic Chemicals on Silver Nanoparticles in Wheat. Int. J. Mol. Sci. 2019, 20, 825.

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