Long Non Coding RNA H19: A New Player in Hypoxia-Induced Multiple Myeloma Cell Dissemination
Abstract
1. Introduction
2. Results
2.1. Hypoxic Stimulation Induced LncH19 Overexpression in MM Cell Models
2.2. LncH19 Sustained Hypoxic Response in MM Cell Lines
2.3. H19 Silencing Affected the Hypoxia-Induced Adhesion of MM Cells on the Stroma
2.4. LncH19 Promoted HIF-1α Activation in Hypoxic MM Cells
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Reagents
4.2. Cell Infection
4.3. RNA Extraction and Real-Time PCR
4.4. Nuclear Protein Extraction and ELISA
4.5. Adhesion Assay
4.6. Total Protein Extract and Western Blot Analysis
4.7. Statistical Analysis
Author Contributions
Funding
Conflicts of Interest
Appendix A
References
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Gene | Primer forward | Primer Reverse |
---|---|---|
LncH19 | GCACCTTGGACATCTGGAGT | TTCTTTCCAGCCCTAGCTCA |
HIF-1α | TGATTGCATCTCCATCTCCTACC | GACTCAAAGCGACAGATAACACG |
VEGF | CGAGGGCCTGGAGTGTGT | CGCATAATCTGCATGGTGATG |
SNAIL | GCGAGCTGCAGGACTCTAAT | CCCGCAATGGTCCACAAAAC |
SLUG | CATGCCTGTCATACCACAAC | GGTGTCAGATGGAGGAGGG |
CXCR4 | TACACCGAGGAAATGGGCTCA | AGATGATGGAGTAGATGGTGG |
B−A TIN | ATCAAGATCATTGCTCCTCCTGA | CTGCTTGCTGATCCACATCTG |
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Corrado, C.; Costa, V.; Giavaresi, G.; Calabrese, A.; Conigliaro, A.; Alessandro, R. Long Non Coding RNA H19: A New Player in Hypoxia-Induced Multiple Myeloma Cell Dissemination. Int. J. Mol. Sci. 2019, 20, 801. https://doi.org/10.3390/ijms20040801
Corrado C, Costa V, Giavaresi G, Calabrese A, Conigliaro A, Alessandro R. Long Non Coding RNA H19: A New Player in Hypoxia-Induced Multiple Myeloma Cell Dissemination. International Journal of Molecular Sciences. 2019; 20(4):801. https://doi.org/10.3390/ijms20040801
Chicago/Turabian StyleCorrado, Chiara, Viviana Costa, Gianluca Giavaresi, Annalisa Calabrese, Alice Conigliaro, and Riccardo Alessandro. 2019. "Long Non Coding RNA H19: A New Player in Hypoxia-Induced Multiple Myeloma Cell Dissemination" International Journal of Molecular Sciences 20, no. 4: 801. https://doi.org/10.3390/ijms20040801
APA StyleCorrado, C., Costa, V., Giavaresi, G., Calabrese, A., Conigliaro, A., & Alessandro, R. (2019). Long Non Coding RNA H19: A New Player in Hypoxia-Induced Multiple Myeloma Cell Dissemination. International Journal of Molecular Sciences, 20(4), 801. https://doi.org/10.3390/ijms20040801