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Int. J. Mol. Sci. 2019, 20(3), 750; https://doi.org/10.3390/ijms20030750

Development of SNP, KASP, and SSR Markers by BSR-Seq Technology for Saturation of Genetic Linkage Map and Efficient Detection of Wheat Powdery Mildew Resistance Gene Pm61

1
The National Engineering Laboratory of Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
2
College of Chemistry and Environment Engineering, Pingdingshan University, Pingdingshan 467000, China
3
College of Life Science and Technology, Harbin Normal University, Harbin 150080, China
4
Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
5
Zhumadian Academy of Agricultural Sciences, Zhumadian 463000, China
*
Authors to whom correspondence should be addressed.
Received: 24 December 2018 / Accepted: 29 January 2019 / Published: 11 February 2019
(This article belongs to the Special Issue Molecular Advances in Wheat and Barley)
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Abstract

The gene Pm61 that confers powdery mildew resistance has been previously identified on chromosome arm 4AL in Chinese wheat landrace Xuxusanyuehuang (XXSYH). To facilitate the use of Pm61 in breeding practices, the bulked segregant analysis-RNA-Seq (BSR-Seq) analysis, in combination with the information on the Chinese Spring reference genome sequence, was performed in the F2:3 mapping population of XXSYH × Zhongzuo 9504. Two single nucleotide polymorphism (SNP), two Kompetitive Allele Specific PCR (KASP), and six simple sequence repeat (SSR) markers, together with previously identified polymorphic markers, saturated the genetic linkage map for Pm61, especially in the proximal side of the target gene that was short of gene-linked markers. In the newly established genetic linkage map, Pm61 was located in a 0.71 cM genetic interval and can be detected in a high throughput scale by the KASP markers Xicsk8 and Xicsk13 or by the standard PCR-based markers Xicscx497 and Xicsx538. The newly saturated genetic linkage map will be useful in molecular marker assisted-selection of Pm61 in breeding for disease resistant cultivar and in its map-based cloning. View Full-Text
Keywords: Triticum aestivum; Landrace; Powdery mildew; Bulked segregant analysis-RNA-Seq (BSR-Seq); Single nucleotide polymorphism (SNP); Kompetitive Allele Specific PCR (KASP) Triticum aestivum; Landrace; Powdery mildew; Bulked segregant analysis-RNA-Seq (BSR-Seq); Single nucleotide polymorphism (SNP); Kompetitive Allele Specific PCR (KASP)
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Hu, J.; Li, J.; Wu, P.; Li, Y.; Qiu, D.; Qu, Y.; Xie, J.; Zhang, H.; Yang, L.; Fu, T.; Yu, Y.; Li, M.; Liu, H.; Zhu, T.; Zhou, Y.; Liu, Z.; Li, H. Development of SNP, KASP, and SSR Markers by BSR-Seq Technology for Saturation of Genetic Linkage Map and Efficient Detection of Wheat Powdery Mildew Resistance Gene Pm61. Int. J. Mol. Sci. 2019, 20, 750.

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