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Int. J. Mol. Sci. 2019, 20(3), 741; https://doi.org/10.3390/ijms20030741

Synthesis of Fe3[email protected] from Pyrolysis of Fe3O4-Lignin Clusters and Its Application for Quick and Sensitive Detection of PrPSc through a Sandwich SPR Detection Assay

College of Materials Science and Engineering, Nanjing Forestry University, Nanjing 210039, China
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Received: 24 December 2018 / Revised: 1 February 2019 / Accepted: 4 February 2019 / Published: 10 February 2019
(This article belongs to the Special Issue Metallomics: Integrated Biosciences for Elements)
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Abstract

The prion protein (PrPSc) has drawn widespread attention due to its pathological potential to cause prion diseases. Herein, we successfully synthesized Fe3[email protected] by carbonizing Fe3O4-lignin clusters, which were prepared through a facile hydrogen bonding interaction between ≡Fe-OH and hydroxyl groups of lignin. Our in-depth investigation confirmed that the composites were Fe3[email protected] core/shell particles. We constructed a novel sandwich surface plasmon resonance (SPR) detection assay for sensitive PrPSc detection, utilizing bare gold surface and aptamer-modified Fe3[email protected] (Fe3[email protected]). Due to the highly specific affinity of Fe3[email protected] towards PrPSc, the sandwich type SPR sensor exhibited excellent analytical performance towards the discrimination and quantitation of PrPSc. A good linear relationship was obtained between the SPR responses and the logarithm of PrPSc concentrations over a range of 0.1–200 ng/mL. The detection sensitivity for PrPSc was improved by ~10 fold compared with the SPR direct detection format. The required detection time was only 20 min. The specificity of the present biosensor was also confirmed by PrPC and other reagents as controls. This proposed approach could also be used to isolate and detect other highly pathogenic biomolecules with similar structural characteristics by altering the corresponding aptamer in the Fe3[email protected] conjugates. View Full-Text
Keywords: surface plasmon resonance; prion protein; aptamer; sensitive; Fe3C; core/shell surface plasmon resonance; prion protein; aptamer; sensitive; Fe3C; core/shell
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Yuan, C.; Lou, Z.; Wang, W.; Yang, L.; Li, Y. Synthesis of Fe3[email protected] from Pyrolysis of Fe3O4-Lignin Clusters and Its Application for Quick and Sensitive Detection of PrPSc through a Sandwich SPR Detection Assay. Int. J. Mol. Sci. 2019, 20, 741.

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