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Antibodies Against the C-Terminus of ApoA-1 Are Inversely Associated with Cholesterol Efflux Capacity and HDL Metabolism in Subjects with and without Type 2 Diabetes Mellitus

1
Department of Endocrinology, University Medical Center Groningen, University of Groningen, 9713 GZ Groningen, The Netherlands
2
Division of Laboratory Medicine, Department of Diagnostics, Hộpitaux Universitaires de Geneve, 1211 Geneva, Switzerland
3
Department of Internal Medicine Specialities, Medical Faculty, Hộpitaux Universitaires de Geneve, 1211 Geneva, Switzerland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(3), 732; https://doi.org/10.3390/ijms20030732
Received: 22 January 2019 / Revised: 6 February 2019 / Accepted: 6 February 2019 / Published: 9 February 2019
(This article belongs to the Section Molecular Pathology, Diagnostics, and Therapeutics)
Background: We determined relationships of cholesterol efflux capacity (CEC), plasma cholesterol esterification (EST) and cholesteryl ester transfer (CET) with anti-c-terminus apoA-1 (Ac-terAA1) and anti-apolipoprotein (apo)-1 (AAA1) autoantibodies in subjects with and without Type 2 diabetes mellitus (T2D). Methods: In 75 T2D subjects and 75 nondiabetic subjects, Ac-terAA1 and AAA1 plasma levels were measured by enzyme-linked immunosorbent assay. CEC was measured as [3H]-cholesterol efflux from human cultured fibroblasts to diluted individual subject plasma. Plasma EST and CET were assayed by isotope methods. Results: Ac-terAA1 and AAA1 levels and were similar between T2D and control subjects. Univariate regression analysis (n = 150) demonstrated that Ac-terAA1 levels were inversely correlated with CEC, EST, CET, total cholesterol, non-HDL cholesterol, triglycerides and apolipoprotein B, (p < 0.05 to p < 0.01), but not with glucose and HbA1c. In separate multivariable linear regression models, CEC, EST and CET were inversely associated with Ac-terAA1 levels independently of age, sex, T2D and drug use (β = −0.186, p = 0.026; β = −0.261, p < 0.001; and β = −0.321, p < 0.001; respectively). These associations were lost after additional adjustment for non-HDL cholesterol and triglycerides. No associations were observed for AAA1. Conclusions: CEC, plasma EST and CET are inversely associated with Ac-terAA1 autoantibodies, conceivably attributable to an inverse relationship of these autoantibodies with apolipoprotein B-containing lipoproteins. View Full-Text
Keywords: anti-apoA-1 autoantibodies; cholesterol efflux capacity; cholesterol esterification; cholesteryl ester transfer; high-density lipoproteins; Type 2 diabetes mellitus anti-apoA-1 autoantibodies; cholesterol efflux capacity; cholesterol esterification; cholesteryl ester transfer; high-density lipoproteins; Type 2 diabetes mellitus
MDPI and ACS Style

Dullaart, R.P.F.; Pagano, S.; Perton, F.G.; Vuilleumier, N. Antibodies Against the C-Terminus of ApoA-1 Are Inversely Associated with Cholesterol Efflux Capacity and HDL Metabolism in Subjects with and without Type 2 Diabetes Mellitus. Int. J. Mol. Sci. 2019, 20, 732. https://doi.org/10.3390/ijms20030732

AMA Style

Dullaart RPF, Pagano S, Perton FG, Vuilleumier N. Antibodies Against the C-Terminus of ApoA-1 Are Inversely Associated with Cholesterol Efflux Capacity and HDL Metabolism in Subjects with and without Type 2 Diabetes Mellitus. International Journal of Molecular Sciences. 2019; 20(3):732. https://doi.org/10.3390/ijms20030732

Chicago/Turabian Style

Dullaart, Robin P.F.; Pagano, Sabrina; Perton, Frank G.; Vuilleumier, Nicolas. 2019. "Antibodies Against the C-Terminus of ApoA-1 Are Inversely Associated with Cholesterol Efflux Capacity and HDL Metabolism in Subjects with and without Type 2 Diabetes Mellitus" Int. J. Mol. Sci. 20, no. 3: 732. https://doi.org/10.3390/ijms20030732

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