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Article

The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method

1
Institute of Clinical Experimental Research, Semmelweis University, Budapest 1094, Hungary
2
Department of Laboratory Medicine, Semmelweis University, Budapest 1089, Hungary
3
Department of Physiology, Semmelweis University, Budapest, 1094 Hungary
4
RT-Europe Non-profit Research Center, Mosonmagyaróvár 9200, Hungary
5
Galenbio Ltd., Mosonmagyaróvár 9200, Hungary
6
Danube University, Center for Regenerative Medicine, Krems an der Donau 3500, Austria
7
Faculdade de Engenharia da Universidade do Porto, Universidade do Porto, Porto 4200-465, Portugal
8
University of Physical Education, Institution of Sport and Health Sciences, Budapest 1123, Hungary
9
Orthosera GmbH, Krems an der Donau 3500, Austria
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(3), 721; https://doi.org/10.3390/ijms20030721
Received: 1 February 2019 / Accepted: 5 February 2019 / Published: 8 February 2019
(This article belongs to the Special Issue Blood-Derived Products for Tissue Repair/Regeneration)
Autologous blood derived products, such as platelet-rich plasma (PRP) and platelet-rich fibrin (PRF) are widely applied in regenerative therapies, in contrast to the drawbacks in their application, mainly deriving from the preparation methods used. Eliminating the disadvantages of both PRP and PRF, hyperacute serum (HAS) opens a new path in autologous serum therapy showing similar or even improved regenerative potential at the same time. Despite the frequent experimental and clinical use of PRP and HAS, their protein composition has not been examined thoroughly yet. Thus, we investigated and compared the composition of HAS, serum, PRP and plasma products using citrate and EDTA by simple laboratory tests, and we compared the composition of HAS, serum, EDTA PRP and plasma by Proteome Profiler and ELISA assays. According to our results the natural ionic balance was upset in both EDTA and citrate PRP as well as in plasma. EDTA PRP contained significantly higher level of growth factors and cytokines, especially platelet derived angiogenic and inflammatory proteins, that can be explained by the significantly higher number of platelets in EDTA PRP. The composition analysis of blood derivatives revealed that although the preparation method of PRP and HAS were similar, the ionic and protein composition of HAS could be advantageous for cell function. View Full-Text
Keywords: hyperacute serum; platelet-rich plasma; blood derived products; composition hyperacute serum; platelet-rich plasma; blood derived products; composition
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MDPI and ACS Style

Kardos, D.; Simon, M.; Vácz, G.; Hinsenkamp, A.; Holczer, T.; Cseh, D.; Sárközi, A.; Szenthe, K.; Bánáti, F.; Szathmary, S.; Nehrer, S.; Kuten, O.; Masteling, M.; Lacza, Z.; Hornyák, I. The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method. Int. J. Mol. Sci. 2019, 20, 721. https://doi.org/10.3390/ijms20030721

AMA Style

Kardos D, Simon M, Vácz G, Hinsenkamp A, Holczer T, Cseh D, Sárközi A, Szenthe K, Bánáti F, Szathmary S, Nehrer S, Kuten O, Masteling M, Lacza Z, Hornyák I. The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method. International Journal of Molecular Sciences. 2019; 20(3):721. https://doi.org/10.3390/ijms20030721

Chicago/Turabian Style

Kardos, Dorottya, Melinda Simon, Gabriella Vácz, Adél Hinsenkamp, Tünde Holczer, Domonkos Cseh, Adrienn Sárközi, Kálmán Szenthe, Ferenc Bánáti, Susan Szathmary, Stefan Nehrer, Olga Kuten, Mariana Masteling, Zsombor Lacza, and István Hornyák. 2019. "The Composition of Hyperacute Serum and Platelet-Rich Plasma Is Markedly Different despite the Similar Production Method" International Journal of Molecular Sciences 20, no. 3: 721. https://doi.org/10.3390/ijms20030721

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