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Article

New Insight on Water Status in Germinating Brassica napus Seeds in Relation to Priming-Improved Germination

1
Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Poznań, ul. Umultowska 89, 61-614 Poznań, Poland
2
NanoBioMedical Centre, Adam Mickiewicz University, Poznań, ul. Umultowska 85, 61-614 Poznań, Poland
3
Groupe de Recherche en Physiologie Végétale (GRPV), Earth and Life Institute–Agronomy (ELI-A), Université catholique de Louvain, Croix du Sud 45, boîte L7.07.13, B-1348 Louvain-la-Neuve, Belgium
*
Author to whom correspondence should be addressed.
Present address: Institute of Biochemistry and Biophysics, Polish Academy of Sciences, ul. Pawińskiego 5A, 02-106 Warsaw, Poland.
Int. J. Mol. Sci. 2019, 20(3), 540; https://doi.org/10.3390/ijms20030540
Received: 18 December 2018 / Revised: 21 January 2019 / Accepted: 25 January 2019 / Published: 28 January 2019
(This article belongs to the Special Issue Seed Development, Dormancy and Germination)
Seed priming is a pre-sowing method successfully used to improve seed germination. Since water plays a crucial role in germination, the aim of this study was to investigate the relationship between better germination performances of osmoprimed Brassica napus seeds and seed water status during germination. To achieve this goal, a combination of different kinds of approaches was used, including nuclear magnetic resonance (NMR) spectroscopy, TEM, and SEM as well as semi-quantitative PCR (semi-qPCR). The results of this study showed that osmopriming enhanced the kinetics of water uptake and the total amount of absorbed water during both the early imbibition stage and in the later phases of seed germination. The spin–spin relaxation time (T2) measurement suggests that osmopriming causes faster water penetration into the seed and more efficient tissue hydration. Moreover, factors potentially affecting water relations in germinating primed seeds were also identified. It was shown that osmopriming (i) changes the microstructural features of the seed coat, e.g., leads to the formation of microcracks, (ii) alters the internal structure of the seed by the induction of additional void spaces in the seed, (iii) increases cotyledons cells vacuolization, and (iv) modifies the expression pattern of aquaporin genes. View Full-Text
Keywords: seed priming; germination; water status; aquaporin genes; NMR spectroscopy; SEM microscopy; TEM microscopy seed priming; germination; water status; aquaporin genes; NMR spectroscopy; SEM microscopy; TEM microscopy
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MDPI and ACS Style

Lechowska, K.; Kubala, S.; Wojtyla, Ł.; Nowaczyk, G.; Quinet, M.; Lutts, S.; Garnczarska, M. New Insight on Water Status in Germinating Brassica napus Seeds in Relation to Priming-Improved Germination. Int. J. Mol. Sci. 2019, 20, 540. https://doi.org/10.3390/ijms20030540

AMA Style

Lechowska K, Kubala S, Wojtyla Ł, Nowaczyk G, Quinet M, Lutts S, Garnczarska M. New Insight on Water Status in Germinating Brassica napus Seeds in Relation to Priming-Improved Germination. International Journal of Molecular Sciences. 2019; 20(3):540. https://doi.org/10.3390/ijms20030540

Chicago/Turabian Style

Lechowska, Katarzyna, Szymon Kubala, Łukasz Wojtyla, Grzegorz Nowaczyk, Muriel Quinet, Stanley Lutts, and Małgorzata Garnczarska. 2019. "New Insight on Water Status in Germinating Brassica napus Seeds in Relation to Priming-Improved Germination" International Journal of Molecular Sciences 20, no. 3: 540. https://doi.org/10.3390/ijms20030540

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