Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s
Abstract
:1. Introduction
2. Results and Discussion
2.1. Preparation of PEPA and PEPA Salts
2.2. The Influence of PEPA Salts on ADSCs’ Adhesion and Proliferation
2.3. Osteogenic Potential of the PEPA Salts
3. Materials and Methods
3.1. Synthesis of PEPA and Metal Salts
3.1.1. General Experimental Remarks
3.1.2. Synthesis of Poly(tBuOEP)
3.1.3. Preparation of the PEPA Solution
3.1.4. Preparation of the Solutions of PEPA Metal Salts
3.2. Cultivation of the ADSCs
3.3. The Study of the Influence of PEPA Salts on Cell Adhesion and Proliferation
3.4. The Study of the Osteogenic Potential of PEPA Salts
3.5. Statistical Analysis
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ADSCs | human adipose-tissue-derived stem cells |
BHT | 2,6-di-tert-butyl-4-methylphenol |
BMP-2 | bone morphogenetic protein 2 |
DSC | differential scanning calorimetry |
GAPDH | glyceraldehyde-3-phosphate dehydrogenase |
MSCs | mesenchymal stem cells |
PEP | poly(ethylene phosphate) |
PEPA | poly(ethylene phosphoric acid) |
PTFE | poly(tetrafluoroethylene) |
RCMG | Research Centre of Medical Genetics |
ROP | ring-opening polymerization |
TGA | thermogravimetric analysis |
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Nifant’ev, I.; Bukharova, T.; Dyakonov, A.; Goldshtein, D.; Galitsyna, E.; Kosarev, M.; Shlyakhtin, A.; Gavrilov, D.; Ivchenko, P. Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s. Int. J. Mol. Sci. 2019, 20, 6242. https://doi.org/10.3390/ijms20246242
Nifant’ev I, Bukharova T, Dyakonov A, Goldshtein D, Galitsyna E, Kosarev M, Shlyakhtin A, Gavrilov D, Ivchenko P. Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s. International Journal of Molecular Sciences. 2019; 20(24):6242. https://doi.org/10.3390/ijms20246242
Chicago/Turabian StyleNifant’ev, Ilya, Tatiana Bukharova, Alexander Dyakonov, Dmitry Goldshtein, Elena Galitsyna, Maxim Kosarev, Andrey Shlyakhtin, Dmitry Gavrilov, and Pavel Ivchenko. 2019. "Osteogenic Differentiation of Human Adipose Tissue-Derived MSCs by Non-Toxic Calcium Poly(ethylene phosphate)s" International Journal of Molecular Sciences 20, no. 24: 6242. https://doi.org/10.3390/ijms20246242