Next Article in Journal
Homologous Recombination under the Single-Molecule Fluorescence Microscope
Previous Article in Journal
RankerGUI: A Computational Framework to Compare Differential Gene Expression Profiles Using Rank Based Statistics
Open AccessArticle

The Effect of Hydroxamic Siderophores Structure on Acetylation of Histone H3 and Alpha Tubulin in Pinus sylvestris Root Cells

Institute of Dendrology, Polish Academy of Sciences, Parkowa 5, 62-035 Kórnik, Poland
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(23), 6099; https://doi.org/10.3390/ijms20236099
Received: 8 October 2019 / Revised: 18 November 2019 / Accepted: 30 November 2019 / Published: 3 December 2019
(This article belongs to the Section Molecular Plant Sciences)
Protein acetylation affects gene expression, as well as other processes in cells, and it might be dependent on the availability of the metals. However, whether iron chelating compounds (siderophores) can have an effect on the acetylation process in plant roots is largely unknown. In the present study, western blotting and confocal microscopy was used to examine the degree of acetylation of histone H3 and alpha tubulin in Pinus sylvestris root cells in the presence of structurally different siderophores. The effect of metabolites that were produced by pathogenic and mycorrhizal fungi was also assessed. No effect was observed on histone acetylation. By contrast, the metabolites of the pathogenic fungus were able to decrease the level of microtubule acetylation, whereas treatment with iron-free ferrioxamine (DFO) was able to increase it. This latter was not observed when ferrioxamine-iron complexes were used. The pathogen metabolites induced important modifications of cytoskeleton organization. Siderophores also induced changes in the tubulin skeleton and these changes were iron-dependent. The effect of siderophores on the microtubule network was dependent on the presence of iron. More root cells with a depolymerized cytoskeleton were observed when the roots were exposed to iron-free siderophores and the metabolites of pathogenic fungi; whereas, the metabolites from mycorrhizal fungi and iron-enriched forms of siderophores slightly altered the cytoskeleton network of root cells. Collectively, these data indicated that the metabolites of pathogenic fungi mirror siderophore action, and iron limitation can lead to enhanced alternations in cell structure and physiology. View Full-Text
Keywords: histone; microtubule; pathogen; mycorrhizal fungi; Scots pine; iron chelating compounds histone; microtubule; pathogen; mycorrhizal fungi; Scots pine; iron chelating compounds
Show Figures

Figure 1

MDPI and ACS Style

Mucha, J.; Pawłowski, T.A.; Klupczyńska, E.A.; Guzicka, M.; Zadworny, M. The Effect of Hydroxamic Siderophores Structure on Acetylation of Histone H3 and Alpha Tubulin in Pinus sylvestris Root Cells. Int. J. Mol. Sci. 2019, 20, 6099.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop