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Article

Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis

by
Wei Ye
,
Taomei Liu
,
Weimin Zhang
*,
Saini Li
,
Muzi Zhu
,
Haohua Li
,
Yali Kong
and
Liqiong Xu
State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangdong Institute of Microbiology, Guangdong Acadamy of Sciences, Guangzhou 510070, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(23), 6090; https://doi.org/10.3390/ijms20236090
Submission received: 24 October 2019 / Revised: 24 November 2019 / Accepted: 28 November 2019 / Published: 3 December 2019
(This article belongs to the Special Issue Wheat and Barley: Acclimatization to Abiotic and Biotic Stress)
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Abstract

Wheat yield is greatly reduced because of the occurrence of leaf spot diseases. Bipolaris sorokiniana is the main pathogenic fungus in leaf spot disease. In this study, B. sorokiniana from wheat leaf (W-B. sorokiniana) showed much stronger pathogenicity toward wheat than endophytic B. sorokiniana from Pogostemon cablin (P-B. sorokiniana). The transcriptomes and metabolomics of the two B. sorokiniana strains and transcriptomes of B. sorokiniana-infected wheat leaves were comparatively analyzed. In addition, the expression levels of unigenes related to pathogenicity, toxicity, and cell wall degradation were predicted and validated by quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis. Results indicated that pathogenicity-related genes, especially the gene encoding loss-of-pathogenicity B (LopB) protein, cell wall-degrading enzymes (particularly glycosyl hydrolase-related genes), and killer and Ptr necrosis toxin-producing related unigenes in the W-B. sorokiniana played important roles in the pathogenicity of W-B. sorokiniana toward wheat. The down-regulation of cell wall protein, photosystem peptide, and rubisco protein suggested impairment of the phytosynthetic system and cell wall of B. sorokiniana-infected wheat. The up-regulation of hydrolase inhibitor, NAC (including NAM, ATAF1 and CUC2) transcriptional factor, and peroxidase in infected wheat tissues suggests their important roles in the defensive response of wheat to W-B. sorokiniana. This is the first report providing a comparison of the transcriptome and metabolome between the pathogenic and endophytic B. sorokiniana strains, thus providing a molecular clue for the pathogenic mechanism of W-B. sorokiniana toward wheat and wheat’s defensive response mechanism to W-B. sorokiniana. Our study could offer molecular clues for controlling the hazard of leaf spot and root rot diseases in wheat, thus improving wheat yield in the future.
Keywords: Bipolaris sorokiniana; comparative transcriptome; pathogenicity; cell wall-degrading enzymes; toxin producing; defensive response Bipolaris sorokiniana; comparative transcriptome; pathogenicity; cell wall-degrading enzymes; toxin producing; defensive response
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MDPI and ACS Style

Ye, W.; Liu, T.; Zhang, W.; Li, S.; Zhu, M.; Li, H.; Kong, Y.; Xu, L. Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis. Int. J. Mol. Sci. 2019, 20, 6090. https://doi.org/10.3390/ijms20236090

AMA Style

Ye W, Liu T, Zhang W, Li S, Zhu M, Li H, Kong Y, Xu L. Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis. International Journal of Molecular Sciences. 2019; 20(23):6090. https://doi.org/10.3390/ijms20236090

Chicago/Turabian Style

Ye, Wei, Taomei Liu, Weimin Zhang, Saini Li, Muzi Zhu, Haohua Li, Yali Kong, and Liqiong Xu. 2019. "Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis" International Journal of Molecular Sciences 20, no. 23: 6090. https://doi.org/10.3390/ijms20236090

APA Style

Ye, W., Liu, T., Zhang, W., Li, S., Zhu, M., Li, H., Kong, Y., & Xu, L. (2019). Disclosure of the Molecular Mechanism of Wheat Leaf Spot Disease Caused by Bipolaris sorokiniana through Comparative Transcriptome and Metabolomics Analysis. International Journal of Molecular Sciences, 20(23), 6090. https://doi.org/10.3390/ijms20236090

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