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Open AccessArticle

PatJAZ6 Acts as a Repressor Regulating JA-Induced Biosynthesis of Patchouli Alcohol in Pogostemon Cablin

1
Joint Laboratory of National Engineering Research Center for the Pharmaceutics of Traditional Chinese Medicines, Key Laboratory of Chinese Medicinal Resource from Lingnan, Ministry of Education, Research Center of Chinese Herbal Resource Science and Engineering, Guangzhou University of Chinese Medicine, Guangzhou University of Chinese Medicine, Guangzhou 510006, China
2
School of Pharmaceutical Sciences, Guangdong Food and Drug Vocational College, Guangzhou 510520, China
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(23), 6038; https://doi.org/10.3390/ijms20236038
Received: 12 October 2019 / Revised: 19 November 2019 / Accepted: 27 November 2019 / Published: 30 November 2019
(This article belongs to the Special Issue Jasmonic Acid Pathway in Plants)
The JASMONATE ZIM DOMAIN (JAZ) proteins act as negative regulators in the jasmonic acid (JA) signaling pathways of plants, and these proteins have been reported to play key roles in plant secondary metabolism mediated by JA. In this study, we firstly isolated one JAZ from P. cablin, PatJAZ6, which was characterized and revealed based on multiple alignments and a phylogenic tree analysis. The result of subcellular localization indicated that the PatJAZ6 protein was located in the nucleus of plant protoplasts. The expression level of PatJAZ6 was significantly induced by the methyl jasmonate (MeJA). Furthermore, by means of yeast two-hybrid screening, we identified two transcription factors that interact with the PatJAZ6, the PatMYC2b1 and PatMYC2b2. Virus-induced gene silencing (VIGS) of PatJAZ6 caused a decrease in expression abundance, resulting in a significant increase in the accumulation of patchouli alcohol. Moreover, we overexpressed PatJAZ6 in P. cablin, which down-regulated the patchoulol synthase expression, and then suppressed the biosynthesis of patchouli alcohol. The results demonstrate that PatJAZ6 probably acts as a repressor in the regulation of patchouli alcohol biosynthesis, contributed to a model proposed for the potential JA signaling pathway in P. cablin. View Full-Text
Keywords: PatJAZ6; jasmonic acid (JA) signaling pathway; Pogostemon cablin; patchouli alcohol; biosynthesis PatJAZ6; jasmonic acid (JA) signaling pathway; Pogostemon cablin; patchouli alcohol; biosynthesis
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Wang, X.; Chen, X.; Zhong, L.; Zhou, X.; Tang, Y.; Liu, Y.; Li, J.; Zheng, H.; Zhan, R.; Chen, L. PatJAZ6 Acts as a Repressor Regulating JA-Induced Biosynthesis of Patchouli Alcohol in Pogostemon Cablin. Int. J. Mol. Sci. 2019, 20, 6038.

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