Next Article in Journal
A Paradigm in Immunochemistry, Revealed by Monoclonal Antibodies to Spatially Distinct Epitopes on Syntenin-1
Next Article in Special Issue
Pairing and Exchanging between Daypyrum villosum Chromosomes 6V#2 and 6V#4 in the Hybrids of Two Different Wheat Alien Substitution Lines
Previous Article in Journal
A Phase II, Double-Blind, Randomized, Placebo-Controlled, Multicenter Study Evaluating the Efficacy and Safety of Alpha-1 Antitrypsin (AAT) (Glassia®) in the Treatment of Recent-Onset Type 1 Diabetes
Previous Article in Special Issue
Species Identification of Oaks (Quercus L., Fagaceae) from Gene to Genome
Open AccessArticle

Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis

by Xiaodong Zhang 1,2,†, Caixia Li 1,2,†, Lianchun Wang 1, Yahong Fei 3 and Wensheng Qin 4,*
1
College of Chemistry Biology and Environment, Yuxi Normal University, Yuxi 653100, China
2
Food and Bioengineering College, Xuchang University, Xuchang 461000, China
3
Yuxi Flyingbear Agricultural Development Company Limited, Yuxi 653100, China
4
Department of Biology, Lakehead University, Thunder Bay, ON P7B 5E1, Canada
*
Author to whom correspondence should be addressed.
These authors contribute to this article equally.
Int. J. Mol. Sci. 2019, 20(23), 6034; https://doi.org/10.3390/ijms20236034
Received: 14 September 2019 / Revised: 21 November 2019 / Accepted: 27 November 2019 / Published: 29 November 2019
(This article belongs to the Special Issue Plant Genomics 2019)
Cardiovascular diseases (CVDs) are a major cause of health loss in the world. Prevention and treatment of this disease by traditional Chinese medicine is a promising method. Centranthera grandiflora Benth is a high-value medicinal herb in the prevention and treatment of CVDs; its main medicinal components include iridoid glycosides, phenylethanoid glycosides, and azafrin in roots. However, biosynthetic pathways of these components and their regulatory mechanisms are unknown. Furthermore, there are no genomic resources of this herb. In this article, we provide sequence and transcript abundance data for the root, stem, and leaf transcriptome of C. grandiflora Benth obtained by the Illumina Hiseq2000. More than 438 million clean reads were obtained from root, stem, and leaf libraries, which produced 153,198 unigenes. Based on databases annotation, a total of 557, 213, and 161 unigenes were annotated to catalpol, acteoside, and azafrin biosynthetic pathways, respectively. Differentially expressed gene analysis identified 14,875 unigenes differentially enriched between leaf and root with 8,054 upregulated genes and 6,821 downregulated genes. Candidate MYB transcription factors involved in catalpol, acteoside, and azafrin biosynthesis were also predicated. This work is the first transcriptome analysis in C. grandiflora Benth which will aid the deciphering of biosynthesis pathways and regulatory mechanisms of active components. View Full-Text
Keywords: Centranthera grandiflora Benth; transcriptome; catalpol biosynthesis; acteoside biosynthesis; azafrin biosynthesis Centranthera grandiflora Benth; transcriptome; catalpol biosynthesis; acteoside biosynthesis; azafrin biosynthesis
Show Figures

Figure 1

MDPI and ACS Style

Zhang, X.; Li, C.; Wang, L.; Fei, Y.; Qin, W. Analysis of Centranthera grandiflora Benth Transcriptome Explores Genes of Catalpol, Acteoside and Azafrin Biosynthesis. Int. J. Mol. Sci. 2019, 20, 6034.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop