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Open AccessArticle

Significant Benefits of Nanoparticles Containing a Necrosis Inhibitor on Mice Testicular Tissue Autografts Outcomes

1
Gynecology-Andrology Unit, Medical School, Institute of Experimental and Clinical Research, Catholic University of Louvain, UCLouvain, 1200 Brussels, Belgium
2
Advanced Drug Delivery and Biomaterials Unit, Louvain Drug Research Institute, Catholic University of Louvain, UCLouvain, 1200 Brussels, Belgium
3
Department of Gynecology-Andrology, Saint-Luc University Hospital, 1200 Brussels, Belgium
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(23), 5833; https://doi.org/10.3390/ijms20235833
Received: 26 October 2019 / Revised: 14 November 2019 / Accepted: 18 November 2019 / Published: 20 November 2019
(This article belongs to the Special Issue Molecular Basis of Fertility Preservation and Restoration)
Fertility preservation for prepubertal boys relies exclusively on cryopreservation of immature testicular tissue (ITT) containing spermatogonia as the only cells with reproductive potential. Preclinical studies that used a nude mice model to evaluate the development of human transplanted ITT were characterized by important spermatogonial loss. We hypothesized that the encapsulation of testicular tissue in an alginate matrix supplemented with nanoparticles containing a necrosis inhibitor (NECINH-NPS) would improve tissue integrity and germ cells’ survival in grafts. We performed orthotopic autotransplantation of 1 mm³ testicular tissue fragments recovered form mice (aged 4–5 weeks). Fragments were either non-encapsulated, encapsulated in an alginate matrix, or encapsulated in an alginate matrix containing NECINH-NPs. Grafts were recovered 5- and 21-days post-transplantation. We evaluated tissue integrity (hematoxylin-eosin staining), germ cells survival (immunohistochemistry for promyelocytic leukemia zinc-finger, VASA, and protein-boule-like), apoptosis (immunohistochemistry for active-caspase 3), and lipid peroxidation (immunohistochemistry for malondialdehyde). NECINH-NPs significantly improved testicular tissue integrity and germ cells’ survival after 21 days. Oxidative stress was reduced after 5 days, regardless of nanoparticle incorporation. No effect on caspase-dependent apoptosis was observed. In conclusion, NECINH-NPs in an alginate matrix significantly improved tissue integrity and germ cells’ survival in grafts with the perspective of higher reproductive outcomes. View Full-Text
Keywords: necrosis inhibitor; nanoparticles; prepubertal testicular tissue; transplantation; fertility preservation; spermatogonia; prepubertal boys; necrosis; tissue engineering; male fertility necrosis inhibitor; nanoparticles; prepubertal testicular tissue; transplantation; fertility preservation; spermatogonia; prepubertal boys; necrosis; tissue engineering; male fertility
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MDPI and ACS Style

Del Vento, F.; Vermeulen, M.; Ucakar, B.; Poels, J.; des Rieux, A.; Wyns, C. Significant Benefits of Nanoparticles Containing a Necrosis Inhibitor on Mice Testicular Tissue Autografts Outcomes. Int. J. Mol. Sci. 2019, 20, 5833. https://doi.org/10.3390/ijms20235833

AMA Style

Del Vento F, Vermeulen M, Ucakar B, Poels J, des Rieux A, Wyns C. Significant Benefits of Nanoparticles Containing a Necrosis Inhibitor on Mice Testicular Tissue Autografts Outcomes. International Journal of Molecular Sciences. 2019; 20(23):5833. https://doi.org/10.3390/ijms20235833

Chicago/Turabian Style

Del Vento, Federico; Vermeulen, Maxime; Ucakar, Bernard; Poels, Jonathan; des Rieux, Anne; Wyns, Christine. 2019. "Significant Benefits of Nanoparticles Containing a Necrosis Inhibitor on Mice Testicular Tissue Autografts Outcomes" Int. J. Mol. Sci. 20, no. 23: 5833. https://doi.org/10.3390/ijms20235833

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