MicroRNA-29a Mitigates Subacromial Bursa Fibrosis in Rotator Cuff Lesion with Shoulder Stiffness
Abstract
:1. Introduction
2. Results
2.1. Subacromial Bursitis in Rotator Cuff Lesion with Shoulder Stiffness
2.2. Decreased miR-29a Expression in Rotator Cuff Lesion with Shoulder Stiffness
2.3. miR-29a Overexpression Attenuated Inflammation and Gait Irregularity
2.4. miR-29a Overexpression Downregulated Fibrotic Matrix Formation
2.5. miR-29 Precursor Treatment Improved Tendon Fibrosis in Injured Shoulders
2.6. miR-29a Directly Targeted COL3A1 Transcription in Inflamed Tenocytes
3. Discussion
4. Materials and Methods
4.1. Patient Recruitment
4.2. Diagnosis of Shoulder Stiffness
4.3. Harvest of Clinical Specimens
4.4. Histomorphometry, In Situ Hybridization, and Immunohistochemistry
4.5. RT-Quantitative PCR
4.6. miR-29a Transgenic Mice
4.7. Shoulder Rotator Cuff Lesion in Mice
4.8. Desktop Digital Ultrasound Analysis
4.9. In Vivo Near-Infrared Fluorescence Assay
4.10. Gait Analysis
4.11. miR-29a Precursor Treatment
4.12. Transfection of Human Tenocyte Cultures
4.13. Luciferase Reporter Assessment of miR-29a Binding to 3′-UTR of COL3A1
4.14. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Stiffness | Non-Stiffness | p Value | |
---|---|---|---|
Participants | 22 | 35 | |
Gender | |||
Female | 19 (86.4%) | 24 (68.6%) | 0.8 |
Male | 3 (13.6%) | 11 (31.4%) | - |
Involved shoulder | |||
Right | 13 (59%) | 24 (68.6%) | 0.78 |
Left | 9 (41%) | 11 (31.4%) | - |
Age (years) | 62.1 ± 2 | 64.8 ± 1.2 | 0.12 |
(48–77) | (52–80) | ||
BMI | 25.1 ± 1 | 25.4 ± 0.5 | 0.42 |
(16.6–32.2) | (20.8–33.4) | ||
Functional score of Constant and Murley | 39.2 ± 2.2 | 63.5 ± 1.5 | <0.001 |
(21–67) | (49–85) | ||
Sum of passive range of motion (SROM) | 196.6 ± 10.1 | 365 ± 9.7 | <0.001 |
(115–270) | (275–520) | ||
Flexion | 83.4 ± 5 | 147.1 ± 3.5 | <0.001 |
(35–130) | (100–180) | ||
Abduction | 72.5 ± 4.8 | 119.7 ± 3.5 | <0.001 |
(40–110) | (75–165) | ||
External rotation | 15.2 ± 2.6 | 41.9 ± 3.1 | <0.001 |
(0–40) | (10–75) | ||
Internal rotation | 26 ± 6 | 54.6 ± 4.6 | <0.001 |
(0–80) | (0–90) |
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Ko, J.-Y.; Lian, W.-S.; Tsai, T.-C.; Chen, Y.-S.; Hsieh, C.-K.; Kuo, C.-W.; Wang, F.-S. MicroRNA-29a Mitigates Subacromial Bursa Fibrosis in Rotator Cuff Lesion with Shoulder Stiffness. Int. J. Mol. Sci. 2019, 20, 5742. https://doi.org/10.3390/ijms20225742
Ko J-Y, Lian W-S, Tsai T-C, Chen Y-S, Hsieh C-K, Kuo C-W, Wang F-S. MicroRNA-29a Mitigates Subacromial Bursa Fibrosis in Rotator Cuff Lesion with Shoulder Stiffness. International Journal of Molecular Sciences. 2019; 20(22):5742. https://doi.org/10.3390/ijms20225742
Chicago/Turabian StyleKo, Jih-Yang, Wei-Shiung Lian, Tsai-Chen Tsai, Yu-Shan Chen, Chin-Kuei Hsieh, Chung-Wen Kuo, and Feng-Sheng Wang. 2019. "MicroRNA-29a Mitigates Subacromial Bursa Fibrosis in Rotator Cuff Lesion with Shoulder Stiffness" International Journal of Molecular Sciences 20, no. 22: 5742. https://doi.org/10.3390/ijms20225742