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Open AccessArticle

Depicting Conformational Ensembles of α-Synuclein by Single Molecule Force Spectroscopy and Native Mass Spectroscopy

1
School of Medicine and Surgery, Nanomedicine Center NANOMIB, University of Milan-Bicocca, 20900 Monza, Italy
2
Department of Materials Science, University of Milan-Bicocca, 20125 Milan, Italy
3
Department of Biotechnology and Biosciences, University of Milan-Bicocca, 20126 Milan, Italy
4
Scuola Internazionale Superiore di Studi Avanzati, SISSA, 34136 Trieste, Italy
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(20), 5181; https://doi.org/10.3390/ijms20205181
Received: 11 September 2019 / Revised: 3 October 2019 / Accepted: 17 October 2019 / Published: 19 October 2019
Description of heterogeneous molecular ensembles, such as intrinsically disordered proteins, represents a challenge in structural biology and an urgent question posed by biochemistry to interpret many physiologically important, regulatory mechanisms. Single-molecule techniques can provide a unique contribution to this field. This work applies single molecule force spectroscopy to probe conformational properties of α-synuclein in solution and its conformational changes induced by ligand binding. The goal is to compare data from such an approach with those obtained by native mass spectrometry. These two orthogonal, biophysical methods are found to deliver a complex picture, in which monomeric α-synuclein in solution spontaneously populates compact and partially compacted states, which are differently stabilized by binding to aggregation inhibitors, such as dopamine and epigallocatechin-3-gallate. Analyses by circular dichroism and Fourier-transform infrared spectroscopy show that these transitions do not involve formation of secondary structure. This comparative analysis provides support to structural interpretation of charge-state distributions obtained by native mass spectrometry and helps, in turn, defining the conformational components detected by single molecule force spectroscopy. View Full-Text
Keywords: α-synuclein; single molecule force spectroscopy; intrinsically disordered proteins; native mass spectrometry α-synuclein; single molecule force spectroscopy; intrinsically disordered proteins; native mass spectrometry
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Corti, R.; Marrano, C.A.; Salerno, D.; Brocca, S.; Natalello, A.; Santambrogio, C.; Legname, G.; Mantegazza, F.; Grandori, R.; Cassina, V. Depicting Conformational Ensembles of α-Synuclein by Single Molecule Force Spectroscopy and Native Mass Spectroscopy. Int. J. Mol. Sci. 2019, 20, 5181.

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