Laser-Driven Ultrashort Pulsed Electron Beam Radiation at Doses of 0.5 and 1.0 Gy Induces Apoptosis in Human Fibroblasts
by
Nelly Babayan 1,2, Bagrat Grigoryan 3, Lusine Khondkaryan 1, Gohar Tadevosyan 1, Natalya Sarkisyan 1, Ruzanna Grigoryan 1, Lilit Apresyan 1, Rouben Aroutiounian 2, Natalia Vorobyeva 4,5, Margarita Pustovalova 6, Anna Grekhova 7 and Andreyan N. Osipov 4,5,6,*
Nelly Babayan 1,2, Bagrat Grigoryan 3, Lusine Khondkaryan 1, Gohar Tadevosyan 1, Natalya Sarkisyan 1, Ruzanna Grigoryan 1, Lilit Apresyan 1, Rouben Aroutiounian 2, Natalia Vorobyeva 4,5, Margarita Pustovalova 6, Anna Grekhova 7 and Andreyan N. Osipov 4,5,6,*
1
Institute of Molecular Biology NAS RA, 7 Hasratyan, 0014 Yerevan, Armenia
2
Yerevan State University, 1 Manoogian, 0025 Yerevan, Armenia
3
CANDLE Synchrotron Research Institute, 31 Acharyan, 0040 Yerevan, Armenia
4
State Research Center - Burnasyan Federal Medical Biophysical Center of Federal Medical Biological Agency, 46 Zhivopisnaya, 123182 Moscow, Russia
5
Semenov Institute of Chemical Physics. Russian Academy of Sciences, 4 Kosygina, Moscow 119991, Russia
6
Moscow Institute of Physics and Technology, Dolgoprudny, Moscow Region 141700, Russia
7
Emanuel Institute for Biochemical Physics. Russian Academy of Sciences, Moscow 119991, Russia
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(20), 5140; https://doi.org/10.3390/ijms20205140
Received: 8 August 2019 / Revised: 9 October 2019 / Accepted: 16 October 2019 / Published: 17 October 2019
(This article belongs to the Collection Radiation Toxicity in Cells)
Rapidly evolving laser technologies have led to the development of laser-generated particle accelerators as an alternative to conventional facilities. However, the radiobiological characteristics need to be determined to enhance their applications in biology and medicine. In this study, the radiobiological effects of ultrashort pulsed electron beam (UPEB) and X-ray radiation in human lung fibroblasts (MRC-5 cell line) exposed to doses of 0.1, 0.5, and 1 Gy are compared. The changes of γH2AX foci number as a marker of DNA double-strand breaks (DSBs) were analyzed. In addition, the micronuclei induction and cell death via apoptosis were studied. We found that the biological action of UPEB-radiation compared to X-rays was characterized by significantly slower γH2AX foci elimination (with a dose of 1 Gy) and strong apoptosis induction (with doses of 0.5 and 1.0 Gy), accompanied by a slight increase in micronuclei formation (dose of 1 Gy). Our data suggest that UPEB radiation produces more complex DNA damage than X-ray radiation, leading to cell death rather than cytogenetic disturbance.
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MDPI and ACS Style
Babayan, N.; Grigoryan, B.; Khondkaryan, L.; Tadevosyan, G.; Sarkisyan, N.; Grigoryan, R.; Apresyan, L.; Aroutiounian, R.; Vorobyeva, N.; Pustovalova, M.; Grekhova, A.; Osipov, A.N. Laser-Driven Ultrashort Pulsed Electron Beam Radiation at Doses of 0.5 and 1.0 Gy Induces Apoptosis in Human Fibroblasts. Int. J. Mol. Sci. 2019, 20, 5140.
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