Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture
Abstract
:1. Introduction
2. Results
2.1. MSC Culture in HS- or FCS -Based Medium
2.2. Transient Serum Exposure Regimes
2.3. Transient Serum Exposure Regimes in Primary MSC Cultures
3. Discussion
3.1. Effect of Serum Regimes on Cell Growth
3.2. Effect of Serum Regimes on Cell Differentiation
3.3. Transient Serum Approaches for Cell Differentiation
4. Materials and Methods
4.1. Cell Culture
4.2. Surface Marker Expression
4.3. Fluorescent Cell Staining
4.4. Apoptosis Assay
4.5. Cell Growth Measurements
4.6. Alizarin Red Staining and Quantification
4.7. Alkaline Phosphatase Assay
4.8. Serum Biochemical Analysis
4.9. RT-PCR
4.10. Statistical Analysis
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
ALP | Alkaline phosphatase |
FCS | Fetal calf serum |
HS | Human serum |
MSC | Mesenchymal stem cell |
PBS | Phosphate-buffered saline |
SC | Standard culture |
Appendix A
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Popov, A.; Scotchford, C.; Grant, D.; Sottile, V. Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture. Int. J. Mol. Sci. 2019, 20, 5051. https://doi.org/10.3390/ijms20205051
Popov A, Scotchford C, Grant D, Sottile V. Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture. International Journal of Molecular Sciences. 2019; 20(20):5051. https://doi.org/10.3390/ijms20205051
Chicago/Turabian StylePopov, Alexander, Colin Scotchford, David Grant, and Virginie Sottile. 2019. "Impact of Serum Source on Human Mesenchymal Stem Cell Osteogenic Differentiation in Culture" International Journal of Molecular Sciences 20, no. 20: 5051. https://doi.org/10.3390/ijms20205051