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Open AccessArticle

Fibrin-Hyaluronic Acid Hydrogel (RegenoGel) with Fibroblast Growth Factor-18 for In Vitro 3D Culture of Human and Bovine Nucleus Pulposus Cells

1
AO Research Institute Davos, 7270 Davos, Switzerland
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Department of Orthopaedic, Inselspital Bern, 3010 Bern, Switzerland
3
Universitätsklinik Wien, 1090 Vienna, Austria
4
ProCore Ltd. Weizmann Science Park, 76400 Ness Ziona, Israel
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(20), 5036; https://doi.org/10.3390/ijms20205036
Received: 13 August 2019 / Revised: 6 October 2019 / Accepted: 9 October 2019 / Published: 11 October 2019
We investigated the effects of a fibrin-hyaluronic acid hydrogel (FBG–HA) and fibroblast growth factor 18 (FGF-18) for nucleus pulposus (NP) regeneration. Healthy bovine (n = 4) and human degenerated NP cells (n = 4) were cultured for 14 days in FBG-HA hydrogel with FGF-18 (∆51-mutant or wild-type) in the culture medium. Gene expression, DNA content, and glycosaminoglycan (GAG) synthesis were evaluated on day 7 and 14. Additionally, histology was performed. Human NP cells cultured in FBG-HA hydrogel showed an increase in collagen type II (COL2) and carbonic anhydrase XII (CA12) gene expression after 14 or 7 days of culture, respectively. GAG release into the conditioned medium increased over 14 days. Healthy bovine NP cells showed increased gene expression of ACAN from day 7 to day 14. Wild type FGF-18 up-regulated CA12 gene expression of human NP cells. Histology revealed an increase of proteoglycan deposition upon FGF-18 stimulation in bovine but not in human NP cells. The FBG-HA hydrogel had a positive modulatory effect on human degenerated NP cells. Under the tested conditions, no significant effect of FGF-18 was observed on cell proliferation or GAG synthesis in human NP cells. View Full-Text
Keywords: fibrin-hyaluronic acid hydrogel; fibroblast growth factor 18; nucleus pulposus cells fibrin-hyaluronic acid hydrogel; fibroblast growth factor 18; nucleus pulposus cells
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Häckel, S.; Zolfaghar, M.; Du, J.; Hoppe, S.; Benneker, L.M.; Garstka, N.; Peroglio, M.; Alini, M.; Grad, S.; Yayon, A.; Li, Z. Fibrin-Hyaluronic Acid Hydrogel (RegenoGel) with Fibroblast Growth Factor-18 for In Vitro 3D Culture of Human and Bovine Nucleus Pulposus Cells. Int. J. Mol. Sci. 2019, 20, 5036.

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