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Open AccessArticle

Integration of Transcriptomics and Metabolomics for Pepper (Capsicum annuum L.) in Response to Heat Stress

1
Longping Branch, Graduate School of Hunan University, Changsha 410125, China
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Hunan Vegetable Research Institute, Changsha 410125, China
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College of Horticulture and Landscape, Hunan Agricultural University, Changsha 410125, China
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Hubei Academy of Agricultural Sciences, Wuhan 430064, China
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Department of Botany, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(20), 5042; https://doi.org/10.3390/ijms20205042
Received: 4 September 2019 / Revised: 4 October 2019 / Accepted: 10 October 2019 / Published: 11 October 2019
(This article belongs to the Special Issue ROS and Abiotic Stress in Plants)
Heat stress (HS), caused by extremely high temperatures, is one of the most severe forms of abiotic stress in pepper. In the present study, we studied the transcriptome and metabolome of a heat-tolerant cultivar (17CL30) and a heat-sensitive cultivar (05S180) under HS. Briefly, we identified 5754 and 5756 differentially expressed genes (DEGs) in 17CL30 and 05S180, respectively. Moreover, we also identified 94 and 108 differentially accumulated metabolites (DAMs) in 17CL30 and 05S180, respectively. Interestingly, there were many common HS-responsive genes (approximately 30%) in both pepper cultivars, despite the expression patterns of these HS-responsive genes being different in both cultivars. Notably, the expression changes of the most common HS-responsive genes were typically much more significant in 17CL30, which might explain why 17CL30 was more heat tolerant. Similar results were also obtained from metabolome data, especially amino acids, organic acids, flavonoids, and sugars. The changes in numerous genes and metabolites emphasized the complex response mechanisms involved in HS in pepper. Collectively, our study suggested that the glutathione metabolic pathway played a critical role in pepper response to HS and the higher accumulation ability of related genes and metabolites might be one of the primary reasons contributing to the heat resistance. View Full-Text
Keywords: transcriptome; metabolome; heat shock protein; heat shock transcription factors; glutathione transcriptome; metabolome; heat shock protein; heat shock transcription factors; glutathione
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Wang, J.; Lv, J.; Liu, Z.; Liu, Y.; Song, J.; Ma, Y.; Ou, L.; Zhang, X.; Liang, C.; Wang, F.; Juntawong, N.; Jiao, C.; Chen, W.; Zou, X. Integration of Transcriptomics and Metabolomics for Pepper (Capsicum annuum L.) in Response to Heat Stress. Int. J. Mol. Sci. 2019, 20, 5042.

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