Next Article in Journal
Comparing the Osteogenic Potentials and Bone Regeneration Capacities of Bone Marrow and Dental Pulp Mesenchymal Stem Cells in a Rabbit Calvarial Bone Defect Model
Previous Article in Journal
Translesion DNA Synthesis Across Lesions Induced by Oxidative Products of Pyrimidines: An Insight into the Mechanism by Microscale Thermophoresis
Open AccessArticle

Genome-Wide Identification of Direct Targets of the TTG1–bHLH–MYB Complex in Regulating Trichome Formation and Flavonoid Accumulation in Arabidopsis Thaliana

by Zelou Wei 1,†, Yalong Cheng 2,3,†, Chenchen Zhou 1, Dong Li 1, Xin Gao 1, Shuoxin Zhang 2,3,* and Mingxun Chen 1,*
1
State Key Laboratory of Crop Stress Biology for Arid Areas and College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China
2
College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi, China
3
Qinling National Forest Ecosystem Research Station, Huoditang, Ningshan 711600, Shaanxi, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(20), 5014; https://doi.org/10.3390/ijms20205014
Received: 6 September 2019 / Revised: 27 September 2019 / Accepted: 8 October 2019 / Published: 10 October 2019
(This article belongs to the Section Molecular Plant Sciences)
Extensive studies have shown that the MBW complex consisting of three kinds of regulatory proteins, MYB and basic helix–loop–helix (bHLH) transcription factors and a WD40 repeat protein, TRANSPARENT TESTA GLABRA1 (TTG1), acts in concert to promote trichome formation and flavonoid accumulation in Arabidopsis thaliana. TTG1 functions as an essential activator in these two biological processes. However, direct downstream targets of the TTG1-dependent MBW complex have not yet been obtained in the two biological processes at the genome-wide level in A. thaliana. In the present study, we found, through RNA sequencing and quantitative real-time PCR analysis, that a great number of regulatory and structural genes involved in both trichome formation and flavonoid accumulation are significantly downregulated in the young shoots and expanding true leaves of ttg1-13 plants. Post-translational activation of a TTG1-glucocorticoid receptor fusion protein and chromatin immunoprecipitation assays demonstrated that these downregulated genes are directly or indirectly targeted by the TTG1-dependent MBW complex in vivo during trichome formation and flavonoid accumulation. These findings further extend our understanding of the role of TTG1-dependent MBW complex in the regulation of trichome formation and flavonoid accumulation in A. thaliana. View Full-Text
Keywords: MBW complex; TTG1; trichome formation; flavonoid accumulation; Arabidopsis thaliana MBW complex; TTG1; trichome formation; flavonoid accumulation; Arabidopsis thaliana
Show Figures

Figure 1

MDPI and ACS Style

Wei, Z.; Cheng, Y.; Zhou, C.; Li, D.; Gao, X.; Zhang, S.; Chen, M. Genome-Wide Identification of Direct Targets of the TTG1–bHLH–MYB Complex in Regulating Trichome Formation and Flavonoid Accumulation in Arabidopsis Thaliana. Int. J. Mol. Sci. 2019, 20, 5014.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop