c-Src Recruitment is Involved in c-MET-Mediated Malignant Behaviour of NT2D1 Non-Seminoma Cells
Abstract: c-MET pathway over-activation is the signature of malignancy acquisition or chemotherapy resistance of many cancers. We recently demonstrated that type II Testicular Germ Cell Tumours (TGCTs) express c-MET receptor. In particular, we elucidated that the non-seminoma lesions express c-MET protein at higher level, compared with the seminoma ones. In line with this observation, NTERA-2 clone D1 (NT2D1) non-seminoma cells increase their proliferation, migration and invasion in response to Hepatocyte Growth Factor (HGF). One of the well-known adaptor-proteins belonging to c-MET signaling cascade is c-Src. Activation of c-Src is related to the increase of aggressiveness of many cancers. For this reason, we focused on the role of c-Src in c-MET-triggered and HGF-dependent NT2D1 cell activities. In the present paper, we have elucidated that this adaptor-protein is involved in HGF-dependent NT2D1 cell proliferation, migration and invasion, since Src inhibitor-1 administration abrogates these responses. Despite these biological evidences western blot analyses have not revealed the increase of c-Src activation because of HGF administration. However, notably, immunofluorescence analyses revealed that cytoplasmic and membrane-associated localization of c-Src shifted to the nuclear compartment after HGF stimulation. These results shed new light in the modality of HGF-dependent c-Src recruitment, and put the basis for novel investigations on the relationship between c-Src, and TGCT aggressiveness.
- Supplementary File 1:
PDF-Document (PDF, 765 KB)
Share & Cite This Article
Leonetti, E.; Gesualdi, L.; Scheri, K.C.; Dinicola, S.; Fattore, L.; Masiello, M.G.; Cucina, A.; Mancini, R.; Bizzarri, M.; Ricci, G.; Catizone, A. c-Src Recruitment is Involved in c-MET-Mediated Malignant Behaviour of NT2D1 Non-Seminoma Cells. Int. J. Mol. Sci. 2019, 20, 320.
Leonetti E, Gesualdi L, Scheri KC, Dinicola S, Fattore L, Masiello MG, Cucina A, Mancini R, Bizzarri M, Ricci G, Catizone A. c-Src Recruitment is Involved in c-MET-Mediated Malignant Behaviour of NT2D1 Non-Seminoma Cells. International Journal of Molecular Sciences. 2019; 20(2):320.Chicago/Turabian Style
Leonetti, Erica; Gesualdi, Luisa; Scheri, Katia Corano; Dinicola, Simona; Fattore, Luigi; Masiello, Maria Grazia; Cucina, Alessandra; Mancini, Rita; Bizzarri, Mariano; Ricci, Giulia; Catizone, Angela. 2019. "c-Src Recruitment is Involved in c-MET-Mediated Malignant Behaviour of NT2D1 Non-Seminoma Cells." Int. J. Mol. Sci. 20, no. 2: 320.
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.