Silkworm Storage Protein 1 Inhibits Autophagy-Mediated Apoptosis
Abstract
:1. Introduction
2. Results
2.1. SP1 Inhibits Cell Death Induced by EBSS Treatment
2.2. Effects of SP1 Expression on Caspase-3 Activation and ROS Generation
2.3. SP1 Inhibits LC3 Conversion
2.4. SP1 Regulates ATG7 Expression Level
2.5. Effect of SP1 on Beclin-1 Expression
2.6. Effect of SP1 on Autophagy Blockade-Induced Cell Death
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Autophagy Induction
4.2. Cell Viability Assay
4.3. Relative Caspase-3 Activity Measurement
4.4. Intracellular ROS Level Measurement
4.5. Real-Time PCR
4.6. Western Blotting Analysis
4.7. Statistical Analysis
Author Contributions
Acknowledgments
Conflicts of Interest
References
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CHO gene | Quantitative real-time polymerase chain reaction (qRT-PCR) primers (5′-3′) |
---|---|
SP1 | Forward: ACA TGA AGA TGA AGG AGC TTT GC |
Reverse: TAG AGA CCC TTG CAG TCG GTT | |
Beclin-1 | Forward: CGA ATG TCA GAA CTA CAA ACG |
Reverse: CAG CCT CTC CTC CTC TAA G | |
Atg7 | Forward: GGG AGA AGA ACC AGA AAG G |
Reverse: GAC CAA TCG CCA ACA CAT | |
β-actin | Forward: AGC TGA GAG GGA AAT TGT GCG |
Reverse: GCA ACG GAA CCG CTC ATT |
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Kang, S.J.; Rhee, W.J. Silkworm Storage Protein 1 Inhibits Autophagy-Mediated Apoptosis. Int. J. Mol. Sci. 2019, 20, 318. https://doi.org/10.3390/ijms20020318
Kang SJ, Rhee WJ. Silkworm Storage Protein 1 Inhibits Autophagy-Mediated Apoptosis. International Journal of Molecular Sciences. 2019; 20(2):318. https://doi.org/10.3390/ijms20020318
Chicago/Turabian StyleKang, Su Jin, and Won Jong Rhee. 2019. "Silkworm Storage Protein 1 Inhibits Autophagy-Mediated Apoptosis" International Journal of Molecular Sciences 20, no. 2: 318. https://doi.org/10.3390/ijms20020318