Next Article in Journal
Growth Factor Quantification of Platelet-Rich Plasma in Burn Patients Compared to Matched Healthy Volunteers
Next Article in Special Issue
The microRNAs Regulating Vascular Smooth Muscle Cell Proliferation: A Minireview
Previous Article in Journal
Molecular Mechanisms of Leucine Zipper EF-Hand Containing Transmembrane Protein-1 Function in Health and Disease
Previous Article in Special Issue
Essential Role of Endothelial MCPIP in Vascular Integrity and Post-Ischemic Remodeling
Article Menu

Export Article

Open AccessArticle
Int. J. Mol. Sci. 2019, 20(2), 287; https://doi.org/10.3390/ijms20020287

Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response

1
Department of Health Science, University of Piemonte Orientale (UPO), via Solaroli 17, 28100 Novara, Italy
2
TissueGraft s.r.l., Spin-off of University of Piemonte Orientale (UPO), via Canobio 4/6, 28100 Novara, Italy
3
ISALIT s.r.l., Spin-off of DISIT, University of Piemonte Orientale (UPO), 15121 Alessandria, Italy
4
Department of Sciences and Technological Innovation, University of Piemonte Orientale (UPO), 15121 Alessandria, Italy
5
Vascular Surgery Unit, Ospedale Maggiore della Carità, 28100 Novara, Italy
*
Author to whom correspondence should be addressed.
Received: 18 December 2018 / Revised: 8 January 2019 / Accepted: 9 January 2019 / Published: 12 January 2019
(This article belongs to the Special Issue Mechanisms of Inflammation in Degenerative Cardiovascular Conditions)
Full-Text   |   PDF [3546 KB, uploaded 12 January 2019]   |  

Abstract

Abdominal aortic aneurysm (AAA) is a focal dilatation of the aorta, caused by both genetic and environmental factors. Although vascular endothelium plays a key role in AAA progression, the biological mechanisms underlying the mechanical stress involvement are only partially understood. In this study, we developed an in vitro model to characterize the role of mechanical stress as a potential trigger of endothelial deregulation in terms of inflammatory response bridging between endothelial cells (ECs), inflammatory cells, and matrix remodeling. In AAA patients, data revealed different degrees of calcification, inversely correlated with wall stretching and also with inflammation and extracellular matrix degradation. In order to study the role of mechanical stimulation, endothelial cell line (EA.hy926) has been cultured in healthy (10% strain) and pathological (5% strain) dynamic conditions using a bioreactor. In presence of tumor necrosis factor alpha (TNF-α), high levels of matrix metalloproteinase-9 (MMP-9) expression and inflammation are obtained, while mechanical stimulation significantly counteracts the TNF-α effects. Moreover, physiological deformation also plays a significant role in the control of the oxidative stress. Overall our findings indicate that, due to wall calcification, in AAA there is a significant change in terms of decreased wall stretching. View Full-Text
Keywords: cardiovascular diseases; abdominal aortic aneurysm; oxidative stress; inflammation; calcification; cyclic stretch cardiovascular diseases; abdominal aortic aneurysm; oxidative stress; inflammation; calcification; cyclic stretch
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
SciFeed

Share & Cite This Article

MDPI and ACS Style

Ramella, M.; Bertozzi, G.; Fusaro, L.; Talmon, M.; Manfredi, M.; Catoria, M.C.; Casella, F.; Porta, C.M.; Boldorini, R.; Fresu, L.G.; Marengo, E.; Boccafoschi, F. Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory Response. Int. J. Mol. Sci. 2019, 20, 287.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top