Glycan Binding Profiling of Jacalin-Related Lectins from the Pteria Penguin Pearl Shell
Abstract
:1. Introduction
2. Results
2.1. Purification and Characterization of Novel Lectins from the Secretory Fluid of Pteria Penguin Mantle
2.2. Amino Acid Sequences of PPL3 and PPL4 Subunits
2.3. Carbohydrate-Binding Profilings of PPL2A, PPL3s, and PPL4
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Isolation of Lectins from the Secretory Fluid of Mantle
4.3. Amino Acid Sequences of PPL3 and PPL4 Subunits
4.4. Sugar-Binding Specificity
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
CAM | S-carboxamidomethylated |
CCA | Cancer antennarius lectin |
CRD | carbohydrate recognition domain |
FAC | frontal affinity chromatography |
GlcNAc | N-acetyl-D-glucosamine |
HPLC | high-performance liquid chromate |
JRL | jacalin-related lectin |
PPL | Pteria penguin lectin |
SDS-PAGE | sodium dodecyl sulfate polyacrylamide gel electrophoresis |
SEM | scanning electron microscopy |
Appendix A
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Saccharides/Glycoproteins | PPL2A a | PPL2B a | PPL3 | PPL4 | |
---|---|---|---|---|---|
mM b | |||||
D-Glucose | >250 | >250 | 200 | 25 | |
D-Fructose | 250 | >250 | 50 | 50 | |
D-Mannose | >250 | >250 | 100 | 25 | |
D-Glucosamine | >250 | >250 | >200 | 200 | |
N-Acetyl-D-glucosamine | 250 | >250 | >200 | 6.25 | |
Trehalose | Glc(α1-1α)Glc | 7.8 | >250 | 25 | 100 |
Kojibiose | Glc(α1-2α)Glc | >250 | 125 | >200 | 100 |
Maltose | Glc(α1-4α)Glc | >250 | >250 | >200 | 200 |
Isomaltose | Glc(α1-6α)Glc | 7.8 | >250 | 12.5 | >200 |
Others c | >250 | >250 | >200 | >200 | |
%(w/v) b | |||||
Chitin oligomers | GlcNAc(β1-4β)GlcNAc | >0.25 | >0.25 | >0.25 | 0.125 |
Chitosan oligomers | GlcNH2(β1-4β)GlcNH2 | >0.25 | >0.25 | >0.25 | >0.25 |
Fetuin | >0.25 | 0.25 | 0.025 | 0.025 | |
Asialo fetuin | 0.078 | 0.078 | 0.0125 | 0.025 | |
Other d | >0.25 | >0.25 | >0.25 | >0.25 |
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Ogawa, T.; Sato, R.; Naganuma, T.; Liu, K.; Lakudzala, A.E.; Muramoto, K.; Osada, M.; Yoshimi, K.; Hiemori, K.; Hirabayashi, J.; et al. Glycan Binding Profiling of Jacalin-Related Lectins from the Pteria Penguin Pearl Shell. Int. J. Mol. Sci. 2019, 20, 4629. https://doi.org/10.3390/ijms20184629
Ogawa T, Sato R, Naganuma T, Liu K, Lakudzala AE, Muramoto K, Osada M, Yoshimi K, Hiemori K, Hirabayashi J, et al. Glycan Binding Profiling of Jacalin-Related Lectins from the Pteria Penguin Pearl Shell. International Journal of Molecular Sciences. 2019; 20(18):4629. https://doi.org/10.3390/ijms20184629
Chicago/Turabian StyleOgawa, Tomohisa, Rie Sato, Takako Naganuma, Kayeu Liu, Agness Ethel Lakudzala, Koji Muramoto, Makoto Osada, Kyosuke Yoshimi, Keiko Hiemori, Jun Hirabayashi, and et al. 2019. "Glycan Binding Profiling of Jacalin-Related Lectins from the Pteria Penguin Pearl Shell" International Journal of Molecular Sciences 20, no. 18: 4629. https://doi.org/10.3390/ijms20184629