Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee Melipona fasciculata
by
Alberto Jorge Oliveira Lopes 1,2,*, Cleydlenne Costa Vasconcelos 1, Francisco Assis Nascimento Pereira 2, Rosa Helena Moraes Silva 1, Pedro Felipe dos Santos Queiroz 1, Caio Viana Fernandes 1, João Batista Santos Garcia 1
, Ricardo Martins Ramos 3, Cláudia Quintino da Rocha 4, Silvia Tereza de Jesus Rodrigues Moreira Lima 1, Maria do Socorro de Sousa Cartágenes 1,* and Maria Nilce de Sousa Ribeiro 2,*
, Ricardo Martins Ramos 3, Cláudia Quintino da Rocha 4, Silvia Tereza de Jesus Rodrigues Moreira Lima 1, Maria do Socorro de Sousa Cartágenes 1,* and Maria Nilce de Sousa Ribeiro 2,*
1
Experimental Study of Pain Laboratory, Department of Physiological Sciences, Federal University of Maranhão, São Luís 65080-805, Brazil
2
Laboratory of Pharmacognosy, Department of Pharmacy, Federal University of Maranhão, São Luís 65080-805, Brazil
3
Research Laboratory Information Systems, Department of Information, Environment, Health and Food Production, Federal Institute of Piauí, Teresina 64000-040, Brazil
4
Chemistry Department, Federal University of Maranhão, São Luís 65080-805, Brazil
*
Authors to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(18), 4512; https://doi.org/10.3390/ijms20184512
Received: 25 August 2019 / Revised: 9 September 2019 / Accepted: 9 September 2019 / Published: 12 September 2019
(This article belongs to the Special Issue Natural Anti-Inflammatory Agents 2019)
The stingless bee, Melipona fasciculata Smith (Apidae, Meliponini), is a native species from Brazil. Their products have high biotechnological potential, however there are no studies about the biological activities of pollen collected by M. fasciculata. In this context, the present study investigated the chemical composition, anti-oxidant, anti-inflammatory, and analgesic activities of hydroethanolic pollen extracts collected by M. fasciculata in three cities in Maranhão State, Brazil. We verified the antioxidant activity of the extracts and inhibitory activity against the cyclooxygenase enzyme using in vitro assays and in allowed to select the extract with higher efficiency to be used on in vivo assays. In these trials, the selected extract showed high anti-inflammatory activity as well as nociceptive effects at central and peripheral level, suggesting that this extract acts on inhibition of histamine release and decreased synthesis of prostaglandins and the in-silico study suggested that polyphenols and acids fatty acids in the extract may be associated with these activities. The results of the present study report the high biological potential of pollen extract and we conclude that the pollen collected by M. fasciculata can be considered as the object of research for new pharmacological alternatives.
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Keywords:
pollen; extract; pain; anti-inflammatory; natural products; molecular docking
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MDPI and ACS Style
Lopes, A.J.O.; Vasconcelos, C.C.; Pereira, F.A.N.; Silva, R.H.M.; Queiroz, P.F.S.; Fernandes, C.V.; Garcia, J.B.S.; Ramos, R.M.; Rocha, C.Q.; Lima, S.T.J.R.M.; Cartágenes, M.S.S.; Ribeiro, M.N.S. Anti-Inflammatory and Antinociceptive Activity of Pollen Extract Collected by Stingless Bee Melipona fasciculata. Int. J. Mol. Sci. 2019, 20, 4512.
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