Next Article in Journal
Molecular Cloning and Functional Identification of a Squalene Synthase Encoding Gene from Alfalfa (Medicago sativa L.)
Previous Article in Journal
MicroRNA Networks Modulate Oxidative Stress in Cancer
Previous Article in Special Issue
Transcriptome Analysis of Chinese Chestnut (Castanea mollissima Blume) in Response to Dryocosmus kuriphilus Yasumatsu Infestation
Open AccessArticle

Genome-Wide Expression Profiling of Genes Associated with the Lr47-Mediated Wheat Resistance to Leaf Rust (Puccinia triticina)

by 1,†, 1,†, 1,†, 1, 3, 1, 1,4, 2,* and 1,*
1
College of Plant Protection, Biological Control Center for Plant Diseases and Plant Pests of Hebei, Hebei Agricultural University, Baoding 071000, Hebei, China
2
State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, No. 2 West Yuanmingyuan Road, Beijing 100193, China
3
College of Life Sciences, Hebei Agricultural University, Baoding 071000, Hebei, China
4
Graduate School of Chinese Academy of Agricultural Sciences, Beijing 100081, China
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(18), 4498; https://doi.org/10.3390/ijms20184498
Received: 26 July 2019 / Revised: 3 September 2019 / Accepted: 4 September 2019 / Published: 11 September 2019
Puccinia triticina (Pt), the causal agent of wheat leaf rust, is one of the most destructive fungal pathogens threatening global wheat cultivations. The rational utilization of leaf rust resistance (Lr) genes is still the most efficient method for the control of such diseases. The Lr47 gene introgressed from chromosome 7S of Aegilops speltoides still showed high resistance to the majority of Pt races collected in China. However, the Lr47 gene has not been cloned yet, and the regulatory network of the Lr47-mediated resistance has not been explored. In the present investigation, transcriptome analysis was applied on RNA samples from three different wheat lines (“Yecora Rojo”, “UC1037”, and “White Yecora”) carrying the Lr47 gene three days post-inoculation with the epidemic Pt race THTT. A comparison between Pt-inoculated and water-inoculated “Lr47-Yecora Rojo” lines revealed a total number of 863 upregulated (q-value < 0.05 and log2foldchange > 1) and 418 downregulated (q-value < 0.05 and log2foldchange < −1) genes. Specifically, differentially expressed genes (DEGs) located on chromosomes 7AS, 7BS, and 7DS were identified, ten of which encoded receptor-like kinases (RLKs). The expression patterns of these RLK genes were further determined by a time-scale qRT-PCR assay. Moreover, heatmaps for the expression profiles of pathogenesis-related (PR) genes and several transcription factor gene families were generated. Using a transcriptomic approach, we initially profiled the transcriptional changes associated with the Lr47-mediated resistance. The identified DEGs, particularly those genes encoding RLKs, might serve as valuable genetic resources for the improvement of wheat resistance to Pt. View Full-Text
Keywords: Transcriptome; receptor-like kinases; transcription factor; wheat; leaf rust Transcriptome; receptor-like kinases; transcription factor; wheat; leaf rust
Show Figures

Figure 1

MDPI and ACS Style

Wu, J.; Gao, J.; Bi, W.; Zhao, J.; Yu, X.; Li, Z.; Liu, D.; Liu, B.; Wang, X. Genome-Wide Expression Profiling of Genes Associated with the Lr47-Mediated Wheat Resistance to Leaf Rust (Puccinia triticina). Int. J. Mol. Sci. 2019, 20, 4498.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop