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Open AccessArticle

A Novel Method for Controlled Gene Expression via Combined Bleomycin and Plasmid DNA Electrotransfer

Biophysical Research Group, Faculty of Natural Sciences, Vytautas Magnus University, Vileikos st. 8, LT-44404 Kaunas, Lithuania
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Int. J. Mol. Sci. 2019, 20(16), 4047; https://doi.org/10.3390/ijms20164047
Received: 15 July 2019 / Revised: 8 August 2019 / Accepted: 17 August 2019 / Published: 19 August 2019
Electrochemotherapy is an efficient method for the local treatment of cutaneous and subcutaneous metastases, but its efficacy as a systemic treatment remains low. The application of gene electrotransfer (GET) to transfer DNA coding for immune system modulating molecules could allow for a systemic effect, but its applications are limited because of possible side effects, e.g., immune system overactivation and autoimmune response. In this paper, we present the simultaneous electrotransfer of bleomycin and plasmid DNA as a method to increase the systemic effect of bleomycin-based electrochemotherapy. With appropriately selected concentrations of bleomycin and plasmid DNA, it is possible to achieve efficient cell transfection while killing cells via the cytotoxic effect of bleomycin at later time points. We also show the dynamics of both cell electrotransfection and cell death after the simultaneous electrotransfer of bleomycin and plasmid DNA. Therefore, this method could have applications in achieving the transient, cell death-controlled expression of immune system activating genes while retaining efficient bleomycin mediated cell killing. View Full-Text
Keywords: gene electrotransfer; cell death; bleomycin; plasmid DNA; transfection; cytotoxic effect gene electrotransfer; cell death; bleomycin; plasmid DNA; transfection; cytotoxic effect
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Chopra, S.; Ruzgys, P.; Jakutaviciute, M.; Rimgailaite, A.; Navickaitė, D.; Satkauskas, S. A Novel Method for Controlled Gene Expression via Combined Bleomycin and Plasmid DNA Electrotransfer. Int. J. Mol. Sci. 2019, 20, 4047.

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