Electrical Stimulation of the Mesencephalic Locomotor Region Has No Impact on Blood–Brain Barrier Alterations after Cerebral Photothrombosis in Rats
Abstract
:1. Introduction
2. Results
2.1. MLR-HFS Does Not Change BBB Leakage and Albumin Extravasation
2.2. MLR-HFS Does Not Influence Degradation of Tight Junction Molecules
2.3. MLR-HFS Does Not Affect Matrix Metalloproteinase-9 Expression after Photothrombosis
3. Discussion
4. Materials and Methods
4.1. Animals
4.2. Induction of Photothrombotic Stroke
4.3. Microelectrode Implantation
4.4. High-Frequency Stimulation of the Mesencephalic Locomotor Region
4.5. Collection of Cerebral Tissue
4.6. Immunohistochemistry
4.7. Western Blot Assays
4.8. Zymography
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Schuhmann, M.K.; Stoll, G.; Papp, L.; Bohr, A.; Volkmann, J.; Fluri, F. Electrical Stimulation of the Mesencephalic Locomotor Region Has No Impact on Blood–Brain Barrier Alterations after Cerebral Photothrombosis in Rats. Int. J. Mol. Sci. 2019, 20, 4036. https://doi.org/10.3390/ijms20164036
Schuhmann MK, Stoll G, Papp L, Bohr A, Volkmann J, Fluri F. Electrical Stimulation of the Mesencephalic Locomotor Region Has No Impact on Blood–Brain Barrier Alterations after Cerebral Photothrombosis in Rats. International Journal of Molecular Sciences. 2019; 20(16):4036. https://doi.org/10.3390/ijms20164036
Chicago/Turabian StyleSchuhmann, Michael K., Guido Stoll, Lena Papp, Arne Bohr, Jens Volkmann, and Felix Fluri. 2019. "Electrical Stimulation of the Mesencephalic Locomotor Region Has No Impact on Blood–Brain Barrier Alterations after Cerebral Photothrombosis in Rats" International Journal of Molecular Sciences 20, no. 16: 4036. https://doi.org/10.3390/ijms20164036