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Proteomic Analysis of Lipid Rafts from RBL-2H3 Mast Cells

Department of Cell and Molecular Biology and Pathogenic Bioagents, Ribeirão Preto Medical School, University of São Paulo, Av. Bandeirantes 3900, Ribeirão Preto, SP, 14049-900, Brazil
Molecular Biology Laboratory, Biological Science Institute, Federal University of Goiás, Samambaia Campus II, ICB2, room 206, Goiânia, GO, 74690-900, Brazil
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(16), 3904;
Received: 8 July 2019 / Revised: 1 August 2019 / Accepted: 8 August 2019 / Published: 11 August 2019
(This article belongs to the Section Biochemistry)
PDF [2428 KB, uploaded 11 August 2019]


Lipid rafts are highly ordered membrane microdomains enriched in cholesterol, glycosphingolipids, and certain proteins. They are involved in the regulation of cellular processes in diverse cell types, including mast cells (MCs). The MC lipid raft protein composition was assessed using qualitative mass spectrometric characterization of the proteome from detergent-resistant membrane fractions from RBL-2H3 MCs. Using two different post-isolation treatment methods, a total of 949 lipid raft associated proteins were identified. The majority of these MC lipid raft proteins had already been described in the RaftProtV2 database and are among highest cited/experimentally validated lipid raft proteins. Additionally, more than half of the identified proteins had lipid modifications and/or transmembrane domains. Classification of identified proteins into functional categories showed that the proteins were associated with cellular membrane compartments, and with some biological and molecular functions, such as regulation, localization, binding, catalytic activity, and response to stimulus. Furthermore, functional enrichment analysis demonstrated an intimate involvement of identified proteins with various aspects of MC biological processes, especially those related to regulated secretion, organization/stabilization of macromolecules complexes, and signal transduction. This study represents the first comprehensive proteomic profile of MC lipid rafts and provides additional information to elucidate immunoregulatory functions coordinated by raft proteins in MCs. View Full-Text
Keywords: lipid rafts; membrane proteins; protein localization; regulated secretion; signaling pathway; proteome; mast cells lipid rafts; membrane proteins; protein localization; regulated secretion; signaling pathway; proteome; mast cells

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Freitas Filho, E.G.; Jaca, L.A.M.; Baeza, L.C.; Soares, C.M.A.; Borges, C.L.; Oliver, C.; Jamur, M.C. Proteomic Analysis of Lipid Rafts from RBL-2H3 Mast Cells. Int. J. Mol. Sci. 2019, 20, 3904.

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