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Anti-Bacterial Effect of CpG-DNA Involves Enhancement of the Complement Systems

1
Department of Microbiology, College of Medicine, Hallym University, Chuncheon 24252, Korea
2
Center for Medical Science Research, College of Medicine, Hallym University, Chuncheon 24252, Korea
3
Department of Biochemistry, College of Natural Sciences, Chungbuk National University, Cheongju 28644, Korea
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(14), 3397; https://doi.org/10.3390/ijms20143397
Received: 27 May 2019 / Revised: 30 June 2019 / Accepted: 9 July 2019 / Published: 10 July 2019
(This article belongs to the Section Molecular Immunology)
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Abstract

CpG-DNA activates the host immune system to resist bacterial infections. In this study, we examined the protective effect of CpG-DNA in mice against Escherichia coli (E. coli) K1 infection. Administration of CpG-DNA increased the survival of mice after E. coli K1 infection, which reduces the numbers of bacteria in the organs. Pre-injection of mice with CpG-DNA before E. coli K1 infection increased the levels of the complement C3 but not C3a and C3b. The survival of the mice after E. coli K1 infection was significantly decreased when the mice were pre-injected with the cobra venom factor (CVF) removing the complement compared to the non-CVF-treated mice group. It suggests that the complement has protective roles against E. coli K1 infection. In addition, the survival of complement-depleted mice was increased by CpG-DNA pre-administration before E. coli K1 infection. Therefore, we suggest that CpG-DNA enhances the anti-bacterial activity of the immune system by augmenting the levels of complement systems after E. coli K1 infection and triggering other factors as well. Further studies are required to investigate the functional roles of the CpG-DNA-induced complement regulation and other factors against urgent bacterial infection. View Full-Text
Keywords: cobra venom factor; complement; CpG-DNA; E. coli K1; infection cobra venom factor; complement; CpG-DNA; E. coli K1; infection
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Kim, T.H.; Park, J.; Kim, D.; Gautam, A.; Akauliya, M.; Kim, J.; Lee, H.; Park, S.; Lee, Y.; Kwon, H.-J. Anti-Bacterial Effect of CpG-DNA Involves Enhancement of the Complement Systems. Int. J. Mol. Sci. 2019, 20, 3397.

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