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Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy

1
Institute for Biophysics, Department of Nanobiotechnology (DNBT), BOKU University for Natural Resources and Life Sciences, Muthgasse 11 (Simon Zeisel Haus), A-1190 Vienna, Austria
2
Research Institute of Molecular Pathology (IMP). Campus-Vienna-Biocenter 1, 1030 Vienna, Austria
3
Cancer Heterogeneity Lab, CIC bioGUNE, Bizkaia Science and Technology Park, 48160 Derio, Spain
4
Department Matemáticas para la Economía y la Empresa, Facultad de Economía, Universidad de Valencia, Avda. Tarongers s/n, 46022 Valencia, Spain
5
CNR Nanotec-Istituto di Nanotecnologia, Polo di Nanotecnologia c/o Campus Ecoteckne, Via Monteroni, 73100 Lecce, Italy
*
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Int. J. Mol. Sci. 2019, 20(13), 3275; https://doi.org/10.3390/ijms20133275
Received: 10 April 2019 / Revised: 28 June 2019 / Accepted: 1 July 2019 / Published: 3 July 2019
(This article belongs to the Special Issue Biomechanics of Cell Membrane)
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Abstract

Atomic force microscopy (AFM) combined with fluorescence microscopy has been used to quantify cytomechanical modifications induced by resveratrol (at a fixed concentration of 50 µM) in a breast cancer cell line (MCF-7) upon temporal variation. Cell indentation methodology has been utilized to determine simultaneous variations of Young’s modulus, the maximum adhesion force, and tether formation, thereby determining cell motility and adhesiveness. Effects of treatment were measured at several time-points (0–6 h, 24 h, and 48 h); longer exposures resulted in cell death. Our results demonstrated that AFM can be efficiently used as a diagnostic tool to monitor irreversible morpho/nano-mechanical changes in cancer cells during the early steps of drug treatment. View Full-Text
Keywords: resveratrol; MCF-7; cytomechanics; atomic force microscopy; force spectroscopy; fluorescence microscopy resveratrol; MCF-7; cytomechanics; atomic force microscopy; force spectroscopy; fluorescence microscopy
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Iturri, J.; Weber, A.; Moreno-Cencerrado, A.; Vivanco, M.D.; Benítez, R.; Leporatti, S.; Toca-Herrera, J.L. Resveratrol-Induced Temporal Variation in the Mechanical Properties of MCF-7 Breast Cancer Cells Investigated by Atomic Force Microscopy. Int. J. Mol. Sci. 2019, 20, 3275.

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