Phytosterols Inhibit Side-Chain Oxysterol Mediated Activation of LXR in Breast Cancer Cells
Abstract
:1. Introduction
2. Results
2.1. PSSs Are Poor Transcriptional Activators of LXRA in Breast Cancer Cell Cultures
2.2. PSSs Impair Side-Chain Oxysterol Mediated Activation of LXRA
2.3. STAN and SITO Inhibit Oxysterol Mediated Activation of the LXR Target Genes ABCA1 and APOE
3. Discussion
4. Materials and Methods
4.1. Cell Culture and Cell Lines
4.2. Drugs and Reagents
4.3. MTT Assays
4.4. Reporter Cell Lines and Luciferase Assays
4.5. mRNA Isolation, Reverse Transcription and qPCR
4.6. Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
Abbreviations
24(S)-OHC | 24(S)-hydroxycholesterol |
24,25-EC | 24,25-epoxycholesterol |
25-OHC | 25-hydroxycholesterol |
(25R)26-OHC | (25R),26-hydroxycholesterol |
BRAS | brassicasterol |
BCa | breast cancer |
CAMP | campesterol |
ER | oestrogen receptor |
LXR | liver X receptor |
PSS | phytosterol/phytostanol |
STIG | stigmasterol |
STAN | sitostanol |
SITO | β-sitosterol |
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Hutchinson, S.A.; Lianto, P.; Moore, J.B.; Hughes, T.A.; Thorne, J.L. Phytosterols Inhibit Side-Chain Oxysterol Mediated Activation of LXR in Breast Cancer Cells. Int. J. Mol. Sci. 2019, 20, 3241. https://doi.org/10.3390/ijms20133241
Hutchinson SA, Lianto P, Moore JB, Hughes TA, Thorne JL. Phytosterols Inhibit Side-Chain Oxysterol Mediated Activation of LXR in Breast Cancer Cells. International Journal of Molecular Sciences. 2019; 20(13):3241. https://doi.org/10.3390/ijms20133241
Chicago/Turabian StyleHutchinson, Samantha A., Priscilia Lianto, J. Bernadette Moore, Thomas A. Hughes, and James L. Thorne. 2019. "Phytosterols Inhibit Side-Chain Oxysterol Mediated Activation of LXR in Breast Cancer Cells" International Journal of Molecular Sciences 20, no. 13: 3241. https://doi.org/10.3390/ijms20133241