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Open AccessArticle

Characterization of a Surface-Active Protein Extracted from a Marine Strain of Penicillium chrysogenum

1
Department of Chemical Sciences, University of Naples (Federico II), Via Cinthia, 80126 Naples, Italy
2
Institute for Microelectronics and Microsystems, Unit of Naples-National Research Council, Via P. Castellino 111, 80127 Naples, Italy
*
Author to whom correspondence should be addressed.
Int. J. Mol. Sci. 2019, 20(13), 3242; https://doi.org/10.3390/ijms20133242
Received: 5 June 2019 / Revised: 26 June 2019 / Accepted: 27 June 2019 / Published: 2 July 2019
(This article belongs to the Collection 20th Anniversary of IJMS: Advances in Biochemistry)
Marine microorganisms represent a reservoir of new promising secondary metabolites. Surface-active proteins with good emulsification activity can be isolated from fungal species that inhabit the marine environment and can be promising candidates for different biotechnological applications. In this study a novel surface-active protein, named Sap-Pc, was purified from a marine strain of Penicillium chrysogenum. The effect of salt concentration and temperature on protein production was analyzed, and a purification method was set up. The purified protein, identified as Pc13g06930, was annotated as a hypothetical protein. It was able to form emulsions, which were stable for at least one month, with an emulsification index comparable to that of other known surface-active proteins. The surface tension reduction was analyzed as function of protein concentration and a critical micellar concentration of 2 μM was determined. At neutral or alkaline pH, secondary structure changes were monitored over time, concurrently with the appearance of protein precipitation. Formation of amyloid-like fibrils of SAP-Pc was demonstrated by spectroscopic and microscopic analyses. Moreover, the effect of protein concentration, a parameter affecting kinetics of fibril formation, was investigated and an on-pathway involvement of micellar aggregates during the fibril formation process was suggested. View Full-Text
Keywords: marine fungi; biosurfactant proteins; amyloid fibrils; emulsions marine fungi; biosurfactant proteins; amyloid fibrils; emulsions
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Cicatiello, P.; Stanzione, I.; Dardano, P.; De Stefano, L.; Birolo, L.; De Chiaro, A.; Monti, D.M.; Petruk, G.; D’Errico, G.; Giardina, P. Characterization of a Surface-Active Protein Extracted from a Marine Strain of Penicillium chrysogenum. Int. J. Mol. Sci. 2019, 20, 3242.

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