High-Fish Oil and High-Lard Diets Differently Affect Testicular Antioxidant Defense and Mitochondrial Fusion/Fission Balance in Male Wistar Rats: Potential Protective Effect of ω3 Polyunsaturated Fatty Acids Targeting Mitochondria Dynamics

Migliaccio, Vincenzo; Sica, Raffaella; Di Gregorio, Ilaria; Putti, Rosalba; Lionetti, Lillà

doi:10.3390/ijms20123110

Submit to this Journal Review for this Journal Edit a Special Issue

Article Menu

...

Open AccessArticle

High-Fish Oil and High-Lard Diets Differently Affect Testicular Antioxidant Defense and Mitochondrial Fusion/Fission Balance in Male Wistar Rats: Potential Protective Effect of ω3 Polyunsaturated Fatty Acids Targeting Mitochondria Dynamics

^1,2,

²,

¹,

² and

^1,*

Department of Chemistry and Biology “Adolfo Zambelli”, University of Salerno, 84084 Fisciano, Italy

Department of Biology, University of Naples, Federico II, 80126 Naples, Italy

Author to whom correspondence should be addressed.

Int. J. Mol. Sci. 2019, 20(12), 3110; https://doi.org/10.3390/ijms20123110

Received: 7 June 2019 / Revised: 23 June 2019 / Accepted: 24 June 2019 / Published: 25 June 2019

(This article belongs to the Special Issue Targeted Protection of Bioactive Compounds to Mitochondria)

View Full-Text Download PDF

Browse Figures

Citation Export

Abstract

High-fat diets rich in fish oil (HFO diet, mainly ω3-PUFAs), in contrast to high-fat diets rich in lard (HL diet, mainly saturated fatty acids) have been shown to induce improvement in mitochondrial function and fusion processes associated with a reduction in reactive oxygen species production in both liver and skeletal muscle. High-fat diets may also impair testicular function, and mitochondria represent important cellular organelles with a pivotal role in reproductive function. Mitochondria are dynamic organelles that frequently undergo fission/fusion processes. A shift toward mitochondrial fusion process has been associated with improvement of mitochondrial function, as well as with ω3-PUFAs protective effects. The present study aimed to analyze the effect of chronic overfeeding (six weeks) with HFO or HL diet on testicular tissue histology, oxidative stress, antioxidant defenses, and mitochondrial fusion (mitofusin 2) and fission (dynamic related protein 1) protein. Our results showed that HFO diet induced less testicular histology impairment, oxidative stress, and apoptosis compared to a HL diet. This finding was associated with an increase in antioxidant activities and a shift toward mitochondrial fusion processes induced by HFO diet compared to HL diet, suggesting that ω3-PUFAs may act as bioactive compound targeting mitochondria dynamics to prevent testicular impairment. View Full-Text

Keywords: ω3 polyunsaturated fatty acids; testis; oxidative stress; apoptosis; MFN2; DRP1

▼ Show Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Share and Cite

MDPI and ACS Style

Migliaccio, V.; Sica, R.; Di Gregorio, I.; Putti, R.; Lionetti, L. High-Fish Oil and High-Lard Diets Differently Affect Testicular Antioxidant Defense and Mitochondrial Fusion/Fission Balance in Male Wistar Rats: Potential Protective Effect of ω3 Polyunsaturated Fatty Acids Targeting Mitochondria Dynamics. Int. J. Mol. Sci. 2019, 20, 3110. https://doi.org/10.3390/ijms20123110

AMA Style

Migliaccio V, Sica R, Di Gregorio I, Putti R, Lionetti L. High-Fish Oil and High-Lard Diets Differently Affect Testicular Antioxidant Defense and Mitochondrial Fusion/Fission Balance in Male Wistar Rats: Potential Protective Effect of ω3 Polyunsaturated Fatty Acids Targeting Mitochondria Dynamics. International Journal of Molecular Sciences. 2019; 20(12):3110. https://doi.org/10.3390/ijms20123110

Chicago/Turabian Style

Migliaccio, Vincenzo, Raffaella Sica, Ilaria Di Gregorio, Rosalba Putti, and Lillà Lionetti. 2019. "High-Fish Oil and High-Lard Diets Differently Affect Testicular Antioxidant Defense and Mitochondrial Fusion/Fission Balance in Male Wistar Rats: Potential Protective Effect of ω3 Polyunsaturated Fatty Acids Targeting Mitochondria Dynamics" International Journal of Molecular Sciences 20, no. 12: 3110. https://doi.org/10.3390/ijms20123110

Find Other Styles

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Metrics

Abstract views Pdf views Html views

Article Menu

High-Fish Oil and High-Lard Diets Differently Affect Testicular Antioxidant Defense and Mitochondrial Fusion/Fission Balance in Male Wistar Rats: Potential Protective Effect of ω3 Polyunsaturated Fatty Acids Targeting Mitochondria Dynamics

Abstract

Share and Cite

Article Metrics

Article Access Statistics

Article Access Map by Country/Region

Further Information

Guidelines

MDPI Initiatives

Follow MDPI